| Replacement of mouse and rat antibody production test; comparison of sensitivity between the in vitro and in vivo methods | |
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MedLine Citation:
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PMID: 12096334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacteria and viruses may be transmitted through contaminated biological materials such as transplantable tumors, cell lines, sera or other biological material. Currently, the screening of biological material is being done using the mouse or rat antibody production (MAP/RAP) test (serological testing). We decided to test and validate an alternative assay using polymerase chain reaction (PCR / RT-PCR) technology to detect viral contamination directly in biological material. The aim of this study therefore is the validation of our new PCR assays and the comparison of PCR and MAP test. For 6/14 viruses the conventional PCR, seems to be more sensitive and more specific in detecting murine viruses. In 12/14 viruses the RT-PCR is more sensitive than MAP-test. In 2/14 cases all detection methods have the same sensitivity. Furthermore, PCR screening clearly contributes to the principles of 3R as a replacement technique as it eliminates the need for using animals to screen for murine viruses in biological material. |
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Authors:
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Frank Bootz; Isabella Sieber |
Publication Detail:
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Type: Comparative Study; English Abstract; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: ALTEX Volume: 19 Suppl 1 ISSN: 1868-596X ISO Abbreviation: ALTEX Publication Date: 2002 |
Date Detail:
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Created Date: 2002-07-03 Completed Date: 2002-10-04 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 100953980 Medline TA: ALTEX Country: Germany |
Other Details:
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Languages: ger Pagination: 76-86 Citation Subset: IM |
Affiliation:
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Institut f?r Labortierkunde Universit?t CH-Z?rich, Switzerland. fobo@ltk.unizh.ch |
Vernacular Title:
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Ersatzmethode f?r den Maus/Ratte Antik?rper Produktions Test; Sensitivit?tsvergleich zwischen in vitro und in vivo Methode. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Testing Alternatives Animals Antibody Formation* Base Sequence DNA Primers Mice Polymerase Chain Reaction / methods* Rats Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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