Document Detail


Replacement of mouse embryonic fibroblasts with bone marrow stromal cells for use in establishing and maintaining embryonic stem cells in mice.
MedLine Citation:
PMID:  22309225     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We attempted to utilize bone marrow stromal cells (BMSCs) as a feeder cells for improving culture efficiency of embryonic stem cells (ESCs). B6CBAF1 blastocysts or ESCs stored after their establishment were seeded onto a feeder layer of either SCA-1+/CD45-/CD11b- BMSCs or mouse embryonic fibroblasts (MEFs). Feeder cell activity in promoting ESC establishment from the blastocysts and in supporting ESC maintenance did not differ significantly between BMSC and MEF feeders. However, the highest efficiency of colony formation after culturing of inner cell mass cells of blastocysts was observed with the BMSC line that secreted the largest amount of leukemia inhibitory factor (LIF). Exogenous LIF was essential for the ESC establishment on BMSC feeder, but not for ESC maintenance. Neither change in stem cell-specific gene expression nor increase in stem cell aneuploidy was detected after the use of BMSC feeder. We conclude that BMSC can be utilized as the feeder of ESC, which improves culture efficiency.
Authors:
Chae Hyun Lee; Jun Hong Park; Jae Hee Lee; Ji-Yeon Ahn; Jong Heum Park; Bo-Ram Lee; Dae Yong Kim; Jeong Mook Lim
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-6
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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