Document Detail


Repetitive domain of Clostridium difficile toxin B exhibits cytotoxic effects on human intestinal epithelial cells and decreases epithelial barrier function.
MedLine Citation:
PMID:  20620216     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
We have used recombinant repetitive domain of Clostridium difficile toxin B obtained from two different strains, rec-TcdB3(10463) and rec-TcdB3(8864) and a model intestinal epithelial cell line(s) to characterize their cytotoxic and cytopathic effect and influence on tight-junction organization. Both recombinant receptor binding domains caused intestinal epithelial cell damage, decreased transepithelial electrical resistance and induced translocation of ZO-1 from tight-junction proteins although less efficiently as holotoxins. Recombinant repetitive TcdB domains also caused stimulation of interleukin IL-8 synthesis in HT-29 cells. This is the first description of glucosyltransferase independent toxicity of TcdB and these C-terminal mediated effects may contribute to the pathophysiology of C. difficile infection.
Authors:
Mateja Zemljic; Maja Rupnik; Melania Scarpa; Gregor Anderluh; Gorgio Palù; Ignazio Castagliuolo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-08
Journal Detail:
Title:  Anaerobe     Volume:  16     ISSN:  1095-8274     ISO Abbreviation:  Anaerobe     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505216     Medline TA:  Anaerobe     Country:  England    
Other Details:
Languages:  eng     Pagination:  527-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Institute of Public Health Maribor, Centre for Microbiology, Research Department, Maribor, Slovenia.
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