| Repetition rate dependency of reactive oxygen species formation during femtosecond laser-based cell surgery. | |
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MedLine Citation:
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PMID: 19895141 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Femtosecond (fs) laser-based cell surgery is typically done in two different regimes, at kHz or MHz repetition rate. Formation of reactive oxygen species (ROS) is an often predicted effect due to illumination with short laser pulses in biological tissue. We present our study on ROS formation in single cells in response to irradiation with fs laser pulses depending on the repetition rate while focusing into the cell nucleus. We observed a significant increase of ROS concentration directly after manipulation followed by a decrease in both regimes at kHz and MHz repetition rate. In addition, effects of consecutive exposures at MHz and kHz repetition rate and vice versa on ROS production were studied. Irradiation with a MHz pulse train followed by a kHz pulse train resulted in a significantly higher increase of ROS concentration than in the reversed case and often caused cell death. In the presence of the antioxidant ascorbic acid, accumulation of ROS and cell death were strongly reduced. Therefore, addition of antioxidants during fs laser-based cell surgery experiments could be advantageous in terms of suppressing photochemical damage to the cell. |
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Authors:
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Judith Baumgart; Kai Kuetemeyer; Willem Bintig; Anaclet Ngezahayo; Wolfgang Ertmer; Holger Lubatschowski; Alexander Heisterkamp |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical optics Volume: 14 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2009 Sep-Oct |
Date Detail:
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Created Date: 2009-11-09 Completed Date: 2010-01-13 Revised Date: 2010-11-22 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 054040 Citation Subset: IM |
Affiliation:
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Laser Zentrum Hannover e.V., Hollerithallee 8, Hannover, 30419, Germany. judith.baumgart@polymtl.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cells, Cultured Dose-Response Relationship, Radiation Endothelial Cells / metabolism*, radiation effects* Laser Therapy / methods* Light Radiation Dosage Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species |
| Comments/Corrections | |
Erratum In:
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J Biomed Opt. 2010 Sep-Oct;15(5):059802 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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