Document Detail

Reperfusion injury induces apoptosis in rabbit cardiomyocytes.
MedLine Citation:
PMID:  7929838     Owner:  NLM     Status:  MEDLINE    
The most effective way to limit myocardial ischemic necrosis is reperfusion, but reperfusion itself may result in tissue injury, which has been difficult to separate from ischemic injury. This report identifies elements of apoptosis (programmed cell death) in myocytes as a response to reperfusion but not ischemia. The hallmark of apoptosis, nucleosomal ladders of DNA fragments (approximately 200 base pairs), was detected in ischemic/reperfused rabbit myocardial tissue but not in normal or ischemic-only rabbit hearts. Granulocytopenia did not prevent nucleosomal DNA cleavage. In situ nick end labeling demonstrated DNA fragmentation predominantly in myocytes. The pattern of nuclear chromatin condensation was distinctly different in reperfused than in persistently ischemic tissue by transmission electron microscopy. Apoptosis may be a specific feature of reperfusion injury in cardiac myocytes, leading to late cell death.
R A Gottlieb; K O Burleson; R A Kloner; B M Babior; R L Engler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  94     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-10     Completed Date:  1994-11-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1621-8     Citation Subset:  AIM; IM    
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92038.
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MeSH Terms
Apoptosis / physiology*
DNA / metabolism
Granulocytes / physiology
Myocardial Ischemia / metabolism,  pathology
Myocardial Reperfusion Injury / pathology*,  physiopathology
Myocardium / metabolism,  pathology*
Nucleosomes / metabolism,  ultrastructure
Grant Support
Reg. No./Substance:
0/Nucleosomes; 9007-49-2/DNA

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