Document Detail

Repeated endothelial removal augments intimal thickening and attenuates EDRF release.
MedLine Citation:
PMID:  7514359     Owner:  NLM     Status:  MEDLINE    
To evaluate the significance of repeated denudation injury in progression of atherosclerosis, we performed a single and then a second balloon denudation on the rabbit carotid arteries. Morphological examinations and organ chamber experiments were performed, and the results were compared. On morphological examinations, reendothelialization was almost completed in 2 wk after redenudation, whereas it required 6 wk after a single denudation. Intimal thickening progressed after redenudation. Organ chamber experiments showed that contractile responses and endothelium-independent relaxation remained unchanged after redenudation. Endothelium-dependent relaxations to acetylcholine, ADP, and substance P decreased progressively by repeating denudation. These relaxation responses were inhibited by NG-nitro-L-arginine, hemoglobin, and methylene blue and were considered to be associated with the production and/or release of endothelium-derived relaxing factor-nitric oxide (EDRF-NO). The diffusion barrier mechanism for the decreased endothelium-dependent relaxations was ruled out using sandwich experiments. In conclusion, repeated endothelial denudation caused progression of intimal thickening and acceleration of endothelial regeneration, and repeated endothelial regeneration resulted in progressively less production and/or release of EDRF-NO.
Y Niimi; H Azuma; K Hirakawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  266     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-06-10     Completed Date:  1994-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1348-56     Citation Subset:  IM    
Department of Neurosurgery, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Acetylcholine / pharmacology
Adenosine Diphosphate / pharmacology
Carotid Arteries / cytology,  drug effects,  physiology
Endothelium, Vascular / cytology,  metabolism*,  physiology*
Microscopy, Electron, Scanning
Nitric Oxide / metabolism*
Substance P / pharmacology
Tunica Intima / physiology*
Reg. No./Substance:
10102-43-9/Nitric Oxide; 33507-63-0/Substance P; 51-84-3/Acetylcholine; 58-64-0/Adenosine Diphosphate

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