Document Detail


Repeated bouts of aerobic exercise enhance regulatory T cell responses in a murine asthma model.
MedLine Citation:
PMID:  19781626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have reported previously that moderate intensity aerobic exercise training attenuates airway inflammation in a murine asthma model. Recent studies implicate regulatory T (Treg) cells in decreasing asthma-related airway inflammation; as such, the current study examined the effect of exercise on Treg cell function in a murine asthma model. Mice were sensitized with ovalbumin (OVA) prior to the start of exercise training at a moderate intensity 3x/week for 4weeks; exercise was performed as treadmill running (13.5m/min, 0% grade). Mice were OVA challenged repeatedly throughout the exercise protocol. At protocol completion, mice were analyzed for changes in the number and suppressive function of CD4(+)CD25(+)Foxp3(+) cells isolated from lungs, mediastinal lymph nodes, and spleens. Results show that exercise increased significantly the number of Foxp3(+) cells within the lungs and mediastinal lymph nodes, but not the spleens, of OVA-treated mice as compared with sedentary controls. Exercise also enhanced the suppression function of CD4(+)CD25(+)Foxp3(+) Treg cells derived from OVA-treated mice as compared with sedentary controls. Specifically, Treg cells from exercised, OVA-treated mice more effectively suppressed CD4(+)CD25(-) cell proliferation and Th2 cytokine production in vitro. Enhanced suppression was associated with increased protein levels of TGF-beta and lesser amounts of IL-10 and IL-17; however, blocking TGF-beta had no effect on suppressive functions. These data demonstrate that exercise-mediated increases in Treg cell function may play a role in the attenuation of airway inflammation. Further, these results indicate that moderate intensity aerobic exercise training may alter the Treg cell function within the asthmatic airway.
Authors:
Thomas Lowder; Kari Dugger; Jessy Deshane; Kim Estell; Lisa M Schwiebert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-09-23
Journal Detail:
Title:  Brain, behavior, and immunity     Volume:  24     ISSN:  1090-2139     ISO Abbreviation:  Brain Behav. Immun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-02-17     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  8800478     Medline TA:  Brain Behav Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-9     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294-0005, USA.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis / physiology*
Animals
Antigens, CD4 / biosynthesis
Asthma / immunology*,  metabolism
Bronchoalveolar Lavage Fluid / cytology
Cell Count
Female
Flow Cytometry
Forkhead Transcription Factors / biosynthesis
Interleukin-10 / biosynthesis
Interleukin-17 / biosynthesis
Interleukin-2 Receptor alpha Subunit / biosynthesis
Lung / metabolism
Lymph Nodes / metabolism
Mice
Mice, Inbred BALB C
Ovalbumin / immunology
Physical Conditioning, Animal / physiology*
T-Lymphocytes, Regulatory / immunology*,  metabolism
Transforming Growth Factor beta / biosynthesis
Grant Support
ID/Acronym/Agency:
1F32HL092726/HL/NHLBI NIH HHS; 1R01HL075465/HL/NHLBI NIH HHS; 5T 32HL007553-25/HL/NHLBI NIH HHS; F32 HL092726-02/HL/NHLBI NIH HHS; R01 HL075465-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD4; 0/Forkhead Transcription Factors; 0/Foxp3 protein, mouse; 0/Interleukin-17; 0/Interleukin-2 Receptor alpha Subunit; 0/Transforming Growth Factor beta; 130068-27-8/Interleukin-10; 9006-59-1/Ovalbumin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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