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Repeated Sprints Alter Signalling Related to Mitochondrial Biogenesis in Humans.
MedLine Citation:
PMID:  22089482     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE;: We investigated the effects of acute and chronic repeated-sprint exercise (RSE) on the skeletal muscle mRNA expression and protein abundance/phosphorylation associated with mitochondrial biogenesis. METHODS;: Ten healthy young adults (7 males, 3 females) performed the RSE trial, comprising of three sets of 5 x 4-s maximal sprints on a non-motorised treadmill, with a 20-s recovery between sprints and 4.5 min between sets. After four weeks of repeated-sprint training, three times per week, participants repeated the RSE. A vastus lateralis muscle biopsy was obtained at rest, immediately after, 1h and 4h after RSE, pre- and post-training. Venous blood lactate and glucose were measured at rest and during recovery. Real time RT-PCR and western blot techniques were used to measure mRNA expression and protein abundance, respectively. RESULTS;: Acute RSE increased the phosphorylation of Acetyl-CoA Carboxylase (ACC; 86%; effect size (ES) 1.4 ± 0.8; p<0.001) and Ca calmodulin-dependent protein kinase II (CaMK II; 69%; ES 0.7 ± 0.6). Peroxisome proliferator-activated receptor γ, co-activator 1α (PGC-1α; 208%; ES 1.5 ± 0.7; p<0.001), and nuclear respiratory factor 1 (NRF1; 92%, ES 0.7 ± 0.8) mRNA expression was increased following RSE. Four weeks of training increased the abundance of PGC-1α protein at rest (33%, ES 0.9 ± 0.7). CONCLUSION;: Both acute and chronic RSE, despite only 60 s and 12 min of exercise respectively, altered the molecular signalling associated with mitochondrial adaptations and PGC-1α mRNA expression in skeletal muscle. However, the small-tomoderate changes in resting PGC-1α protein abundance after training, together with the absence of changes in aerobic fitness, require further research to understand the functional significance of PGC-1α in response to repeated-sprint exercise.
Authors:
Fabio R Serpiello; Michael J McKenna; David J Bishop; Robert J Aughey; Marissa K Caldow; David Cameron-Smith; Nigel K Stepto
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-5
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  -     ISSN:  1530-0315     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Institute of Sport, Exercise and Active Living (ISEAL), 2School of Sport and Exercise Science, Victoria University, Melbourne, Australia; 3Department of Neurological, Neuropsychological, Morphological and Movement Sciences, University of Verona, Italy; 4Western Bulldogs Football Club, Melbourne, Australia; 5Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Faculty of Health, Medicine, Nursing and Behavioural Sciences, Deakin University, Melbourne, Australia; 6Liggins Institute, The University of Auckland, New Zealand.
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