Document Detail

Repairing large radial nerve defects by acellular nerve allografts seeded with autologous bone marrow stromal cells in a monkey model.
MedLine Citation:
PMID:  20701436     Owner:  NLM     Status:  MEDLINE    
In this study, we aimed to evaluate the potential of tissue-engineered nerve grafts created from acellular allogenic nerve tissues combined with autologous bone marrow stromal cells (BMSCs) for repairing large peripheral nerve lesions. In a rhesus monkey model, a 2.5-cm-long gap was created in the radial nerve, followed by implantation of either autografts or acellular allografts seeded with autologous BMSCs, Schwann cells (SCs), or no cells. Five months after surgery nerve regeneration was assessed functionally, electrophysiologically, and histomorphometrically. Compared to non-cell-laden allografts, BMSC-laden allografts remarkably facilitated the recovery of the grasping functions of the animals. This functional improvement was coupled with increased nerve conduction velocities and peak amplitudes of compound motor action potentials, and greater axon growth, as well as higher target muscle weight. Moreover, the intensities of nerve regeneration in the BMSC-laden group were comparable to those achieved with SC-laden allografts and autografts. Our data highlight the potential of BMSC-seed allografts for the repair of long peripheral nerve lesions, and reveal comparable regeneration intensities achieved by BMSC-, and SC-laden allografts, as well as autografts. Given their wide availability, BMSCs may represent a promising cell source for tissue-engineered nerve grafts.
Dong Wang; Xiao-Lin Liu; Jia-Kai Zhu; Jun Hu; Li Jiang; Yang Zhang; Li-Min Yang; Hong-Gang Wang; Qing-Tang Zhu; Jian-Hua Yi; Ting-Fei Xi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurotrauma     Volume:  27     ISSN:  1557-9042     ISO Abbreviation:  J. Neurotrauma     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8811626     Medline TA:  J Neurotrauma     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1935-43     Citation Subset:  IM    
Department of Orthopedic and Microsurgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
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MeSH Terms
Action Potentials / physiology
Analysis of Variance
Bone Marrow Cells / physiology
Bone Marrow Transplantation*
Hand Strength / physiology
Macaca mulatta
Nerve Regeneration / physiology*
Radial Nerve / physiopathology*
Recovery of Function
Schwann Cells / physiology,  transplantation*
Stromal Cells / physiology,  transplantation*
Transplantation, Homologous

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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