Document Detail

Repair of spinal cord transection and its effects on muscle mass and myosin heavy chain isoform phenotype.
MedLine Citation:
PMID:  17717118     Owner:  NLM     Status:  MEDLINE    
A number of significant advances have been developed for treating spinal cord injury during the past two decades. The combination of peripheral nerve grafts and acidic fibroblast growth factor (hereafter referred to as PNG) has been shown to partially restore hindlimb function. However, very little is known about the effects of such treatments in restoring normal muscle phenotype. The primary goal of the current study was to test the hypothesis that PNG would completely or partially restore 1) muscle mass and muscle fiber cross-sectional area and 2) the slow myosin heavy chain phenotype of the soleus muscle. To test this hypothesis, we assigned female Sprague-Dawley rats to three groups: 1) sham control, 2) spinal cord transection (Tx), and 3) spinal cord transection plus PNG (Tx+PNG). Six months following spinal cord transection, the open-field test was performed to assess locomotor function, and then the soleus muscles were harvested and analyzed. SDS-PAGE for single muscle fiber was used to evaluate the myosin heavy chain (MHC) isoform expression pattern following the injury and treatment. Immunohistochemistry was used to identify serotonin (5-HT) fibers in the spinal cord. Compared with the Tx group, the Tx+PNG group showed 1) significantly improved Basso, Beattie, and Bresnahan scores (hindlimb locomotion test), 2) less muscle atrophy, 3) a higher percentage of slow type I fibers, and 4) 5-HT fibers distal to the lesion site. We conclude that the combined treatment of PNG is partially effective in restoring the muscle mass and slow phenotype of the soleus muscle in a T-8 spinal cord-transected rat model.
Yu-Shang Lee; Ching-Yi Lin; Vincent J Caiozzo; Richard T Robertson; Jen Yu; Vernon W Lin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-08-23
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  103     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-29     Completed Date:  2008-01-08     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1808-14     Citation Subset:  IM    
Department of Anatomy & Neurobiology, University of California, Irvine, CA, USA.
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MeSH Terms
Body Weight
Disease Models, Animal
Fibroblast Growth Factor 1 / pharmacology*,  therapeutic use
Intercostal Nerves / transplantation*
Motor Activity / drug effects
Muscle Fibers, Skeletal / drug effects*,  metabolism,  pathology
Muscle Fibers, Slow-Twitch / drug effects,  metabolism
Muscle, Skeletal / drug effects,  innervation,  metabolism,  pathology,  physiopathology
Myosin Heavy Chains / metabolism*
Nerve Regeneration / drug effects
Organ Size
Protein Isoforms / metabolism
Rats, Sprague-Dawley
Recovery of Function
Serotonin / metabolism
Spinal Cord Injuries / drug therapy*,  metabolism,  pathology,  physiopathology,  surgery*
Time Factors
Grant Support
Reg. No./Substance:
0/Myosin Heavy Chains; 0/Protein Isoforms; 104781-85-3/Fibroblast Growth Factor 1; 50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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