| Repair of rat calvarial bone defects by controlled release of rhBMP-2 from an injectable bone regeneration composite. | |
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MedLine Citation:
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PMID: 21916016 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The objective of the present study was to enhance the regeneration ability of an injectable bone regeneration composite (IBRC) by the controlled release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The IBRC comprised nano-hydroxyapatite/collagen (nHAC) particles in an alginate hydrogel carrier. First, bovine serum albumin (BSA) as a model protein was released from IBRC to evaluate its release rules. The results suggested that IBRC is a good controlled release carrier for BSA in the range 5-75 µg/ml. In the in vitro study the rhBMP-2 released from IBRC was determined by an enzyme-linked immunosorbent assay specific for rhBMP-2. The bioactivity of the released rhBMP-2 was evaluated through differentiated function of marrow mesenchymal stem cells (MSCs), as measured by alkaline phosphatase activity. The results of an in vitro study confirmed that rhBMP-2 released continuously for 21 days, and its bioactivity was well preserved during this period. The bone formation ability was assessed using a rat calvarial defect model of critical size. Micro-computed tomography (micro-CT) and histological analysis demonstrated that the IBRC had good bone formation ability, which was promoted through rhBMP-2 released from IBRC/rhBMP-2. In vitro and in vivo studies suggested that the present system is a potential bone critical defect repair material for clinical applications. Copyright © 2011 John Wiley & Sons, Ltd. |
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Authors:
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Rongwei Tan; Zhending She; Mingbo Wang; Xing Yu; He Jin; Qingling Feng |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-13 |
Journal Detail:
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Title: Journal of tissue engineering and regenerative medicine Volume: - ISSN: 1932-7005 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101308490 Medline TA: J Tissue Eng Regen Med Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
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State Key Laboratory of New Ceramics and Fine Processing, Department of Materials Science and Engineering, Tsinghua University, Beijing, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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