| Repair in haploid male germ cells occurs late in differentiation as chromatin is condensing. | |
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MedLine Citation:
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PMID: 12684825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Huntington's Disease (HD) is one of eight progressive neurodegenerative disorders in which the underlying mutation is a CAG expansion encoding a polyglutamine tract. The mechanism of trinucleotide expansion remains poorly understood. We have followed heritable changes in CAG length in male transgenic mice. In germ cells, expansion is limited to the post-meiotic, haploid cell and therefore cannot involve mitotic replication or recombination between a homologous chromosome and a sister chromatid. Expansion occurs by gap filling synthesis when DNA loops comprising the CAG trinucleotide repeats are sealed into the DNA strand. Our data support a model in which expansion occurs late in male germ cell development as spermatids are entering the epididymis at a time when chromatin is condensing. These data indicate that repair can be carried out in germ cells as long as the DNA is accessible. The capacity for repair of germ cells may have important implications for future gene therapy. |
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Authors:
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C T McMurray; I V Kortun |
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Publication Detail:
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Type: Journal Article; Review Date: 2003-04-04 |
Journal Detail:
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Title: Chromosoma Volume: 111 ISSN: 0009-5915 ISO Abbreviation: Chromosoma Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-13 Completed Date: 2004-02-11 Revised Date: 2005-11-16 |
Medline Journal Info:
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Nlm Unique ID: 2985138R Medline TA: Chromosoma Country: Germany |
Other Details:
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Languages: eng Pagination: 505-8 Citation Subset: IM |
Affiliation:
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Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA. mcmurray.cynthia@mayo.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatin / genetics*, physiology DNA / physiology DNA Damage DNA Repair / genetics*, physiology Humans Male Mice Mice, Transgenic Spermatogenesis / physiology* Spermatozoa / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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