Document Detail


Repair of diaphyseal bone defects with calcitriol-loaded PLGA scaffolds and marrow stromal cells.
MedLine Citation:
PMID:  17394384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcitriol (1,25(OH)2D3)-loaded porous poly(D,L-lactide-co-glycolide) (PLGA) scaffolds prepared by solvent casting/salt leaching method were used to repair a 1.5 cm diaphyseal segmental bone defect as a fully absorbable osteogenic biomaterial. The in vitro release of sulforhodamine B (SRB) from PLGA scaffold was measured using spectrophotometer, considering SRB as a model drug. The SRB released from SRB-incorporated PLGA scaffold during 3 months was with relatively low initial burst. The calcitriol-loaded PLGA scaffolds with or without marrow stromal cells (MSCs) were implanted in a critical-sized intercalated bone defect in rabbit femur. Defects were assessed by radiographs until 9 weeks. The bony union of the defect was observed only in the calcitriol-loaded groups. RT-PCR results indicated that MSCs, which were seeded into calcitriol-loaded scaffold, expressed an increased level of alkaline phosphatase, osteonectin, and type I collagen mRNA at day 10. After 2 and 4 weeks, the implanted scaffolds were evaluated by histology. New osteoid matrix and direct calcium deposits were more evident in calcitriol/PLGA/MSC group. Three-dimensional computed tomography and frontal tomographic images of repaired femur showed that normal femur anatomy had been restored with cortical bone with no implanted PLGA remnants at 20 weeks. It can be concluded that the porous calcitriol-loaded PLGA scaffold combined with MSCs may be a novel method for repairing the large loaded bone defect.
Authors:
Sun Jung Yoon; Ki Suk Park; Moon Suk Kim; John M Rhee; Gilson Khang; Hai Bang Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering     Volume:  13     ISSN:  1076-3279     ISO Abbreviation:  Tissue Eng.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-08     Completed Date:  2007-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505538     Medline TA:  Tissue Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1125-33     Citation Subset:  IM    
Affiliation:
BK-21 Polymer Fusion Research Team, Chonbuk National University, Jeonju, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Absorbable Implants*
Animals
Antigens, Differentiation / biosynthesis
Bone Marrow Cells / cytology*
Bone Regeneration*
Bone Substitutes / therapeutic use
Calcification, Physiologic
Calcitriol*
Delayed-Action Preparations
Diaphyses / injuries
Drug Implants / therapeutic use
Femur / injuries*,  metabolism,  radiography
Lactic Acid*
Polyglycolic Acid*
Polymers*
Rabbits
Stromal Cells / cytology
Time Factors
Tomography, X-Ray Computed
Chemical
Reg. No./Substance:
0/Antigens, Differentiation; 0/Bone Substitutes; 0/Delayed-Action Preparations; 0/Drug Implants; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 32222-06-3/Calcitriol; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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