| Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells. | |
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MedLine Citation:
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PMID: 3796587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Xeroderma pigmentosum (XP) is an autosomal recessive human disease, characterized by an extreme sensitivity to sunlight, caused by the inability of cells to repair UV light-induced damage to DNA. Cell fusion was used to transfer fragments of Chinese hamster ovary (CHO) chromosomes into XP cells. The hybrid cells exhibited UV resistance and DNA repair characteristics comparable to those expressed by CHO cells, and their DNA had greater homology with CHO DNA than did the DNA from XP cells. Control experiments consisted of fusion of irradiated and unirradiated XP cells and repeated exposure of unfused XP cells to UV doses used for hybrid selection. These treatments did not result in an increase in UV resistance, repair capability, or homology with CHO DNA. The hybrid cell lines do not, therefore, appear to be XP revertants. The establishment of these stable hybrid cell lines is an initial step toward identifying and cloning CHO DNA repair genes that complement the XP defect in human cells. The method should also be applicable to cloning genes for other diseases, such as ataxia-telangiectasia and Fanconi's anemia. |
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Authors:
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D Karentz; J E Cleaver |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 6 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1986 Oct |
Date Detail:
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Created Date: 1987-02-10 Completed Date: 1987-02-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3428-32 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion Cell Line Cell Survival / radiation effects Cricetinae Cricetulus DNA Repair* / radiation effects DNA Replication / radiation effects Dose-Response Relationship, Radiation Female Humans Hybrid Cells / cytology, radiation effects* Kinetics Mutation* Nucleic Acid Hybridization Ovary Ultraviolet Rays* Xeroderma Pigmentosum / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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5 T32 ES07106/ES/NIEHS NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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