Document Detail

Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells.
MedLine Citation:
PMID:  22551484     Owner:  NLM     Status:  Publisher    
In this study, we investigate the effects of treatment with layered chondrocyte sheets and synovial cell transplantation. An osteochondral defect was created of 48 Japanese white rabbits. In order to determine the effects of treatment, the following 6 groups were produced: (A) synovial cells (1.8 × 10(6) cells), (B)layered chondrocyte sheets (1.7 × 10(6) cells), (C) synovial cells (3.0 × 10(5) cells) + layered chondrocyte sheets, (D)synovial cells (6.0 × 10(5) cells) + layered chondrocyte sheets, (E)synovial cells (1.2 × 10(6) cells) + layered chondrocyte sheets, (F) osteochondral defect. Layered chondrocyte sheets and synovial cells were transplanted, sacrificed four and 12 weeks postoperatively. An incapacitance tester (Linton) was used to find trends in the weight distribution ratio of the damaged limbs after surgery. Sections were stained with Safranin-O. Repair sites were evaluated using ICRS grading system. In groups (A) to (E), the damaged limb weight distribution ratio had improved. The repair tissue stained positively with Safranin-O. Four and 12 weeks after surgery, groups (A) to (E) exhibited significantly higher scores than group (F), and groups (D) and (E) exhibited significantly higher scores than groups (A) and (B). This suggests the efficacy of combining layered chondrocyte sheets with synovial cells.
Satoshi Ito; Masato Sato; Masayuki Yamato; Genya Mitani; Toshiharu Kutsuna; Toshihiro Nagai; Taku Ukai; Miyuki Kobayashi; Mami Kokubo; Teruo Okano; Joji Mochida
Related Documents :
25339644 - Sf treg cells transcribing high levels of bcl-2 and microrna-21 demonstrate limited apo...
21269444 - In planta expression of a. cellulolyticus cel5a endocellulase reduces cell wall recalci...
25418474 - Oxyphil cell metaplasia in the parathyroids is characterized by somatic mitochondrial d...
25437044 - An assessment of norepinephrine mediated hypertrophy to apoptosis transition in cardiac...
24397934 - Structural characterization of the cell division cycle in strigomonas culicis, an endos...
22285464 - The regulation of focal adhesion complex formation and salivary gland epithelial cell o...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-30
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Low-doses aspirin doesn't increase surgical bleedind nor transfusion rate in total knee arthroplasty...
Next Document:  Characterization of natural, decellularized and reseeded porcine tooth bud matrices.