Document Detail


Repair of the injured adult heart involves new myocytes potentially derived from resident cardiac stem cells.
MedLine Citation:
PMID:  21454756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: The ability of the adult heart to generate new myocytes after injury is not established.
OBJECTIVE: Our purpose was to determine whether the adult heart has the capacity to generate new myocytes after injury, and to gain insight into their source.
METHODS AND RESULTS: Cardiac injury was induced in the adult feline heart by infusing isoproterenol (ISO) for 10 days via minipumps, and then animals were allowed to recover for 7 or 28 days. Cardiac function was measured with echocardiography, and proliferative cells were identified by nuclear incorporation of 5-bromodeoxyuridine (BrdU; 7-day minipump infusion). BrdU was infused for 7 days before euthanasia at days 10, 17, and 38 or during injury and animals euthanized at day 38. ISO caused reduction in cardiac function with evidence of myocyte loss from necrosis. During this injury phase there was a significant increase in the number of proliferative cells in the atria and ventricle, but there was no increase in BrdU+ myocytes. cKit+ cardiac progenitor cells were BrdU labeled during injury. During the first 7 days of recovery there was a significant reduction in cellular proliferation (BrdU incorporation) but a significant increase in BrdU+ myocytes. There was modest improvement in cardiac structure and function during recovery. At day 38, overall cell proliferation was not different than control, but increased numbers of BrdU+ myocytes were found when BrdU was infused during injury.
CONCLUSIONS: These studies suggest that ISO injury activates cardiac progenitor cells that can differentiate into new myocytes during cardiac repair.
Authors:
David Angert; Remus M Berretta; Hajime Kubo; Hongyu Zhang; Xiongwen Chen; Wei Wang; Barbara Ogorek; Mary Barbe; Steven R Houser
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2011-03-31
Journal Detail:
Title:  Circulation research     Volume:  108     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-13     Completed Date:  2011-07-18     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1226-37     Citation Subset:  IM    
Affiliation:
Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Cardiomyopathies / pathology*,  physiopathology,  therapy*
Cats
Cell Differentiation / drug effects,  physiology
Isoproterenol / toxicity
Myocytes, Cardiac / cytology*,  drug effects,  pathology
Recovery of Function / drug effects,  physiology
Stem Cells / cytology*,  drug effects,  pathology
Grant Support
ID/Acronym/Agency:
HL033921/HL/NHLBI NIH HHS; HL089312/HL/NHLBI NIH HHS; HL091799/HL/NHLBI NIH HHS; HL095322/HL/NHLBI NIH HHS; R01 HL033921-24/HL/NHLBI NIH HHS; R01 HL088243/HL/NHLBI NIH HHS; R01 HL089312-05/HL/NHLBI NIH HHS; R01 HL090979-01/HL/NHLBI NIH HHS; R37 HL033921/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
7683-59-2/Isoproterenol
Comments/Corrections
Comment In:
Circ Res. 2011 May 13;108(10):1158-9   [PMID:  21566219 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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