| Reoxygenation injury in isolated hepatocytes: cell death precedes conversion of xanthine dehydrogenase to xanthine oxidase. | |
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MedLine Citation:
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PMID: 3166376 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reoxygenation of isolated hepatocytes from fed rats after 3 h of anaerobic incubation led to a significantly enhanced loss of cell viability. No evidence for the participation of reactive oxygen species generated by xanthine oxidase in this reoxygenation injury was found. Conversion of xanthine dehydrogenase to xanthine oxidase occurred at a time when almost all of the hepatocytes had lost their viability. Furthermore, xanthine dehydrogenase was first released from the severely injured cells and then converted to the oxidase form. The results suggest that in the intact organ participation of reactive oxygen species, generated by xanthine oxidase, in reoxygenation injury may only occur when, upon reoxygenation, hypoxic cell injury in part of the tissue has progressed to such an extent that there is a significant conversion of xanthine dehydrogenase to xanthine oxidase. |
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Authors:
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H de Groot; A Littauer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 155 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-10-05 Completed Date: 1988-10-05 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 278-82 Citation Subset: IM |
Affiliation:
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Institut für Physiologische Chemie I, Universität Düsseldorf, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Animals Cell Separation Cell Survival* Ketone Oxidoreductases / metabolism* Liver / enzymology*, metabolism, pathology Male Oxidation-Reduction Rats Rats, Inbred Strains Time Factors Xanthine Dehydrogenase / metabolism* Xanthine Oxidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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EC 1.17.1.4/Xanthine Dehydrogenase; EC 1.17.3.2/Xanthine Oxidase; EC 1.2.-/Ketone Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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