Document Detail


Reoxygenation-induced cell damage of isolated neonatal rat ventricular myocytes can be reduced by chain-breaking antioxidants.
MedLine Citation:
PMID:  8299987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the role of chain-breaking antioxidants on reperfusion injury in the ischemic heart, cultured ventricular heart cells (myocytes) were subjected to hypoxia and reoxygenation. The myocytes were prepared from neonatal rats and cultured in F10 medium that was supplemented with serum. As a marker for cell damage, lactate dehydrogenase was analyzed in the medium. Cells subjected to hypoxia for 5 h showed a 1.9 fold increase in lactate dehydrogenase (LD) leakage, while cells subjected to 1 h hypoxia followed by 4 h reoxygenation showed a 5-fold increase in LD intake. Alpha-tocopherol, beta-carotene, nordihydroguairetic acid (NDGA), butylated hydroxyltoluene (BHT), and ICI211965 were added to the cell medium every 24 h for 6 d prior to reoxygenation. All compounds protected against reoxygenation-induced cell damage. In the presence of the 5-lipoxygenase inhibitor ICI211965, protection against LD leakage was found only at high concentrations, which corresponded to the antioxidative effect of ICI211965, and not to inhibition of 5-lipoxygenase. We conclude that cultured ventricular myocytes can be used to evaluate the protective effect of antioxidants on reoxygenation-induced cell damage, and that chain-breaking antioxidants protected well against reoxygenation-induced cell damage.
Authors:
B Ek; C Hallberg; K G Sjögren; A Hjalmarson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  16     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-10     Completed Date:  1994-03-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-21     Citation Subset:  IM    
Affiliation:
ASTRA HASSLE AB, Mölndal, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn*
Antioxidants*
Butylated Hydroxytoluene / pharmacology
Carotenoids / pharmacology
Cells, Cultured
Heart Ventricles
L-Lactate Dehydrogenase / metabolism
Myocardial Reperfusion Injury / prevention & control*
Myocardium / enzymology
Naphthalenes / pharmacology
Nordihydroguaiaretic Acid / pharmacology
Oxygen / administration & dosage,  pharmacology*
Rats
Rats, Wistar
Thiazoles / pharmacology
Vitamin E / pharmacology
beta Carotene
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Naphthalenes; 0/Thiazoles; 128-37-0/Butylated Hydroxytoluene; 129424-08-4/ICI 211965; 1406-18-4/Vitamin E; 36-88-4/Carotenoids; 500-38-9/Nordihydroguaiaretic Acid; 7235-40-7/beta Carotene; 7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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