| Reovirus-induced sigma1s-dependent G(2)/M phase cell cycle arrest is associated with inhibition of p34(cdc2). | |
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MedLine Citation:
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PMID: 11462015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Serotype 3 reoviruses inhibit cellular proliferation by inducing a G(2)/M phase cell cycle arrest. Reovirus-induced G(2)/M phase arrest requires the viral S1 gene-encoded sigma1s nonstructural protein. The G(2)-to-M transition represents a cell cycle checkpoint that is regulated by the kinase p34(cdc2). We now report that infection with serotype 3 reovirus strain Abney, but not serotype 1 reovirus strain Lang, is associated with inhibition and hyperphosphorylation of p34(cdc2). The sigma1s protein is necessary and sufficient for inhibitory phosphorylation of p34(cdc2), since a viral mutant lacking sigma1s fails to hyperphosphorylate p34(cdc2) and inducible expression of sigma1s is sufficient for p34(cdc2) hyperphosphorylation. These studies establish a mechanism by which reovirus can perturb cell cycle regulation. |
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Authors:
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G J Poggioli; T S Dermody; K L Tyler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 75 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-07-19 Completed Date: 2001-08-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 7429-34 Citation Subset: IM |
Affiliation:
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Department of Microbiology, and Neurology Service, Denver Veterans Affairs Medical Center, Denver, Colorado 80220, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CDC2 Protein Kinase / physiology* Cell Cycle* Cell Line Mammalian orthoreovirus 3 / physiology* Mice Reoviridae Infections / pathology, virology* Signal Transduction Viral Nonstructural Proteins / physiology* Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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AG14071/AG/NIA NIH HHS; AI38296/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Viral Nonstructural Proteins; 0/sigma 1NS protein, Reovirus; EC 2.7.11.22/CDC2 Protein Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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