Document Detail


Renibacterium salmoninarum: effect of hypochlorite treatment, and survival in water.
MedLine Citation:
PMID:  15212289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of different concentrations of sodium hypochlorite on Renibacterium salmoninarum and the survival of the bacterium in autoclaved river water and groundwater were examined. The disinfection trial was performed using R. salmoninarum ATCC 33209. The concentrations of free chlorine were 10, 50, 100 and 200 mg 1(-1), the contact times were 5, 15, and 30 min and 24 h, and the test suspensions were subcultured both on Kidney disease medium (KDM2) agar and in 3 parallel KDM2 broths, which were then subcultured on KDM2 and selective KDM (SKDM) agar. The survival of the bacterium in river water and groundwater was studied using 4 isolates of R. salmoninarum including ATCC 33209. Treatment with sodium hypochlorite effectively reduced the number of culturable cells of R. salmoninarum, but use of the recovery broth showed that small numbers of cells remained viable at all concentrations of free chlorine. The numbers of R. salmoninarum decreased to an undetectable level after 4 wk incubation in the survival trials, but low numbers of colonies were again found in the subculture after 5 wk incubation. Viable cells of R. salmoninarum were still detected in subcultures of all strains after 20 wk of incubation in river water.
Authors:
Varpu Hirvelä-Koski
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  59     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-06-23     Completed Date:  2004-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Affiliation:
National Veterinary and Food Research Institute, Oulu Regional Unit, PO Box 517, 90101 Oulu, Finland. varpu.hirvela-koski@eela.fi
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MeSH Terms
Descriptor/Qualifier:
Agar
Aquaculture
Culture Media
Disinfectants / toxicity*
Dose-Response Relationship, Drug
Finland
Hypochlorous Acid / toxicity*
Micrococcaceae / drug effects*
Rivers / microbiology
Chemical
Reg. No./Substance:
0/Culture Media; 0/Disinfectants; 7790-92-3/Hypochlorous Acid; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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