| Rengyolone inhibits apoptosis via etoposide-induced caspase downregulation. | |
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MedLine Citation:
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PMID: 19349754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the course of screening for substances inhibiting apoptosis of U937 human leukemia cells induced by etoposide (10 microg/ml), Forsythiae fructus, which showed a high level of inhibition, was selected. The regulating compounds were purified from the ethyl acetate extract by silica gel column chromatography and HPLC. The active substance was purified and identified as rengyolone by spectroscopic methods. This compound showed inhibitory activity on caspase-3 induction, a major protease of the apoptosis cascade, with an IC50 value of 38.96 microM after 8 h of etoposide treatment in U937 cells. The expression level of caspase-3 and poly(ADP-ribose) polymerase (PARP) were dose-dependently inhibited by the compound, suggesting that rengyolone inhibits etoposide-induced apoptosis via downregulation of caspases. |
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Authors:
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Jin Hee Kim; Choong Hwan Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microbiology and biotechnology Volume: 19 ISSN: 1017-7825 ISO Abbreviation: J. Microbiol. Biotechnol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-07 Completed Date: 2009-06-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9431852 Medline TA: J Microbiol Biotechnol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 286-90 Citation Subset: IM |
Affiliation:
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Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-dong, Yusong, Daejon 305-764, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology Apoptosis / drug effects* Caspase 3 / antagonists & inhibitors, biosynthesis* DNA Fragmentation / drug effects Dose-Response Relationship, Drug Down-Regulation / drug effects* Enzyme Induction / drug effects Etoposide / pharmacology* Furans / chemistry, isolation & purification, pharmacology* Heterocyclic Compounds, 2-Ring / chemistry, isolation & purification, pharmacology* Humans Plants, Medicinal / chemistry Poly(ADP-ribose) Polymerases / biosynthesis U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Furans; 0/Heterocyclic Compounds, 2-Ring; 0/Rengyolone; 33419-42-0/Etoposide; EC 2.4.2.30/PARP1 protein, human; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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