Document Detail


Renewal of the epithelium in the descending colon of the mouse. IV. Cell population kinetics of vacuolated-columnar and mucous cells.
MedLine Citation:
PMID:  170817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proliferation and migration of cells in the vacuolated-columnar and mucous cell lines were studied in the descending colon of adult female mice given a single injection or a continuous infusion of 3H-thymidine and killed at various intervals from one hour to 12 days. This investigation was carried out using one mum-thick Epon sections which were radioautographed after staining with the periodic acid-Schiff technique and iron-hematoxylin. In the normalized crypts with ten equal segments, labeled vacuolated cells at one hour after injection of 3H-thymidine were encountered in the lower four segments and in decreasing numbers in segments 5 through 7. From the percent labeled cells in segments of the crypt, the birth rate and fluxes of cells were computed. Moreover, it was found that a cell in the vacuolated-columnar cell line would undergo three mitotic cycles on the average from its birth at the cryptal base to its extrusion from the surface; of these three cycles, the last one which took place from segment 3 to segment 7 appeared to be a changeover from dividing cells to non-dividing cells, in accordance with the "slow cut-off" model of Cairnie et al. ('65b). Mucous cells located in segments 1 through 6 of the crypt were capable of incorporating 3H-thymidine and thus capable of undergoing mitosis. However, the rate of turnover of mucous cells based on proliferative rate was found to be much lower than the rate of turnover of mucous cells based on the transit time in the non-dividing segments of the crypt. Since there was a concomitant overproduction of cells in the vacuolated cells and newly formed mucous cells in the lower portion of the crypt, it was concluded that some vacuolated cells would give rise to mucous cells. This putative transformation occurred in the lower four segments of the crypt. Mucous cells which were formed by transformation would migrate upward along the cryptal wall and accumulate more mucus in the theca; in doing so, they would undergo two divisions, on the average, before they became non-dividing mucous cells. In ascending the cryptal walls, both vacuolated-columnar cells and mucous cells appeared to migrate at a similar speed; they moved much slower at the base of the crypt and accelerated toward the upper portion of the crypt, but they migrated at a constant speed in the non-dividing segments of the crypt.
Authors:
W W Chang; N J Nadler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of anatomy     Volume:  144     ISSN:  0002-9106     ISO Abbreviation:  Am. J. Anat.     Publication Date:  1975 Sep 
Date Detail:
Created Date:  1975-12-11     Completed Date:  1975-12-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376312     Medline TA:  Am J Anat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  39-56     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Movement
Colon / cytology*,  metabolism
Female
Inclusion Bodies
Kinetics
Mice / anatomy & histology*
Mitosis
Mucous Membrane / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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