| Renal vasoactive mediator generation in portal hypertensive and bile duct ligated rats. | |
| | |
MedLine Citation:
|
PMID: 8738735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND/METHODS: Vasoactive substances may have a role in the pathogenesis of functional renal abnormalities in patients with cirrhosis. We determined renal vasoactive mediators in rats with portal hypertension since the balance in each part of the kidney between the vasodilator activity of prostaglandin E2 and the vasospastic activity of thromboxane A2, leukotriene B4, leukotriene C4, endothelin-1 and platelet activating factor may determine renal function. Rats with partial portal vein ligation (n = 7), complete bile duct ligation (n = 6) and sham operated (n = 10) were studied. Three weeks following surgery renal function tests, including fractional excretion of sodium [Fe(Na)] were measured. Rats were anesthetized, splenic pulp pressure was measured, kidneys were removed, and cortex, medulla and papilla were separated and homogenized for determination of prostaglandin E2, thromboxane B2, leukotriene B4, leukotriene C4 and endothelin-1 by radioimmunoassay (ng/g) and platelet activating factor activity (pg/10 mg) by platelet aggregation. RESULTS: Pulp pressure was > 13 mmHg in portal vein ligated and bile duct ligated and 6 mmHg in sham operated rats. In bile duct ligated rats there was a 70% decrease in Fe(Na) and a significant decrease in cortical and papillary prostaglandin E2, whereas cortical thromboxane B2 and platelet activating factor activity in the cortex, medulla and papilla were double that of sham operated rats. A similar but insignificant trend of changes was found in portal vein ligated rats. Medullary leukotriene B4 was significantly decreased in bile duct ligated rats. Papillary leukotriene B4 was not detected in bile duct ligated and portal vein ligated rats. Renal leukotriene C4 generation in the three groups was either unchanged (papilla) or beyond detection (cortex and medulla). Medullary and papillary endothelin-1 in portal vein ligated and bile duct ligated rats were 178%-130% higher than in sham operated rats. A significant negative correlation was found between Fe(Na) and cortical and medullary thromboxane B2 generation and medullary platelet activating factor activity. CONCLUSIONS: 1) In bile duct ligated rats enhanced intrarenal generation of thromboxane A2 and platelet activating factor may contribute to decreased renal sodium excretion. 2) The role of decreased intrarenal prostaglandin E2 and increased intrarenal endothelin-1 content in bile duct ligated rats is not yet understood. 3) Renal leukotriene generation is either decreased or undetected in portal vein ligated and bile duct ligated rats. |
| | |
Authors:
|
Z Ackerman; F Karmeli; G Amir; D Rachmilewitz |
Related Documents
:
|
15986875 - A preliminary study of applying chemical biliary duct embolization to chemical hepatect... 2338825 - Development of bioartificial liver: bilirubin conjugation in gunn rats. 10216285 - Bile diversion in rats leads to a decreased plasma concentration of linoleic acid which... 7206785 - Cannulation of the cervical thoracic duct in the rat. 2778665 - Improved anticonvulsant activity of phenytoin by a redox brain delivery system i: synth... 8042005 - Polyploid accumulation associated with hyper-phosphorylation of histone h1 in hepatocyt... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of hepatology Volume: 24 ISSN: 0168-8278 ISO Abbreviation: J. Hepatol. Publication Date: 1996 Apr |
Date Detail:
|
Created Date: 1996-12-11 Completed Date: 1996-12-11 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: DENMARK |
Other Details:
|
Languages: eng Pagination: 478-86 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Hadassah University Hospital, Mount Scopus, Hebrew University Hadassah Medical School, Jerusalem, Israel. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Bile Ducts* Eicosanoids / metabolism Endothelin-1 / metabolism Hemodynamics Hypertension, Portal / metabolism*, physiopathology Kidney / pathology, physiopathology Kidney Function Tests Ligation* Male Platelet Activating Factor / metabolism Rats Rats, Sprague-Dawley Renal Circulation / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Eicosanoids; 0/Endothelin-1; 0/Platelet Activating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phagocytic function and metabolite production in thioacetamide-induced liver cirrhosis: a comparativ...
Next Document: Hyporeactivity of mesenteric resistance arteries in portal hypertensive rats.