| Renal tubular handling of p-aminohippurate and epidermal growth factor (EGF) in filtering and nonfiltering perfused rat kidneys. | |
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MedLine Citation:
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PMID: 1553353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the integrity of renal tubular function in filtering and nonfiltering isolated perfused rat kidneys by using p-amino-3H-hippurate (3H-PAH) and the multiple indicator dilution method with 14C-creatinine as a reference. The influx clearance (PSu,1) of unbound 3H-PAH was 0.37 and 0.38 ml/sec in the filtering and nonfiltering kidneys, respectively. The efflux rate constants were comparable between filtering and nonfiltering kidneys, while the sequestration rate constant in the filtering kidney was approximately three times larger than that in the nonfiltering kidney. These data suggest that the nonfiltering kidney maintains 3H-PAH transporting ability through the antiluminal plasma membrane. The renal handling of epidermal growth factor (EGF) by filtering and nonfiltering kidneys was compared. The ratio of the total uptake of tracer 125I-EGF over 20 min in the nonfiltering kidney to that in the filtering kidney was 0.8. This ratio was reduced to 0.2 when the kidneys were perfused with tracer 125I-EGF plus 20 nM EGF. Furthermore, the total uptake of tracer 125I-EGF in the nonfiltering kidney was reduced 20-fold in the presence of 20 nM unlabeled EGF. These findings suggest that the tubular uptake of tracer 125I-EGF by filtering kidney takes place mainly via the antiluminal plasma membrane and that this uptake is a saturable process. |
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Authors:
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D C Kim; Y Sugiyama; Y Sawada; M Hanano |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pharmaceutical research Volume: 9 ISSN: 0724-8741 ISO Abbreviation: Pharm. Res. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-04-24 Completed Date: 1992-04-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 271-5 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Creatinine / metabolism Dye Dilution Technique Epidermal Growth Factor / metabolism* Iodine Radioisotopes / diagnostic use Kidney / metabolism* Kidney Tubules / metabolism* Male Perfusion Protein Binding Rats Rats, Inbred Strains p-Aminohippuric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Iodine Radioisotopes; 60-27-5/Creatinine; 61-78-9/p-Aminohippuric Acid; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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