| Renal sugar transport in the winter flounder: V. secretion of 2-deoxy-D-galactose. | |
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MedLine Citation:
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PMID: 25586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated renal tubules and renal clearance techniques were used to characterize the renal handling of 2-deoxy-D-galactose (2-d-Gal) by the winter flounder (Pseudopleuronectes americanus). In vitro, energy-dependent, pH-sensitive uptake of 2-d-Gal (2-100 micron) was seen at the antiluminal face of the cell. Clearance measurements showed net secretion of 2-d-Gal in vivo. The mean clearance of 2-d-Gal in 18 fish was 0.98 +/- 0.16 ml/h while the glomerular filtration rate (GFR) was only 0.37 +/- 0.10 ml/h. Secretion was associated with marked renal accumulation of both 2-d-Gal and phosphorylated derivatives (2-d-Gal-1-phosphate). Tissue-to-plasma ratios (T/P) averaged 19 for free sugar and 59 for total sugar. Both clearance ratio and T/P were reduced to approximately 1 by injection of galactose (2.5 mmol/kg) simultaneously with 2-d-Gal (25 mumol/kg). Phlorizin (2.5 mumol/kg) increased net 2-d-Gal secretion, whereas glucose (2.5 mmol/kg) produced no change in secretion. Both compounds depressed 2-d-Gal T/P. This result suggests the presence of readsorptive transport at the brush border, sensitive to glucose and phlorizin. |
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Authors:
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J B Pritchard; G Booz; A Kleinzeller |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 234 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-06-28 Completed Date: 1978-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F424-31 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Animals Biological Transport, Active / drug effects Dinitrophenols / pharmacology Fishes / metabolism* Fucose / metabolism* Galactose / pharmacology Glucose / pharmacology Hydrogen-Ion Concentration Kidney Tubules / metabolism* Metabolic Clearance Rate Osmolar Concentration Phlorhizin / pharmacology Probenecid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Dinitrophenols; 26566-61-0/Galactose; 3713-31-3/Fucose; 50-99-7/Glucose; 57-66-9/Probenecid; 60-81-1/Phlorhizin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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