| Renal metabolism of formic acid 2-[4-(5-nitro-2-furyl)-2-thiazolyl]-hydrazide. | |
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MedLine Citation:
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PMID: 3180335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Formic acid 2-[4-(5-nitro-2-furyl)-2-thiazolyl]-hydrazide (FNT) is a potent renal carcinogen in the rat. This study assessed the metabolism of FNT by the isolated perfused rat kidney and whole rat. The glomerular filtration rate and the fractional excretion of sodium for the isolated perfused kidney indicated that under the conditions of these experiments FNT did not alter these renal parameters. The half-life (t1/2) for FNT in the isolated perfused kidney was 67 +/- 8 min. Using HPLC, a metabolite of FNT was observed in urine from the isolated perfused kidney. This metabolite had absorbance at 385 nm but not 254 nm and could not be detected electrochemically at +500 mV. While the excretion of FNT decreased with time of perfusion, the metabolite excretion increased. Whole animal studies demonstrated that FNT is rapidly cleared from blood within the first 5 min of administration. The FNT metabolite was excreted at approximately the same rate from 0-30 and 30-60 min after FNT administration. The metabolite was not observed in media from FNT perfused kidneys or plasma from animals administered FNT. Analysis of purified metabolite by liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS) determined the structure to be 5-nitro-2-furonitrile. This structure assignment was verified by chemical synthesis. Results demonstrate target organ metabolism of carcinogen. |
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Authors:
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S Ballal; M B Mattammal; V M Lakshmi; L A Spry; T V Zenser; B B Davis |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carcinogenesis Volume: 9 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1988 Nov |
Date Detail:
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Created Date: 1988-12-09 Completed Date: 1988-12-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1975-9 Citation Subset: IM |
Affiliation:
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Geriatric Research, Education and Clinical Center (GRECC), Veterans Administration Medical Center, St Louis, MO 63125. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Kidney / metabolism* Nitrofurans / metabolism*, urine Perfusion Rats Rats, Inbred Strains Spectrum Analysis Xenobiotics / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA-28015/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nitrofurans; 0/Xenobiotics; 3570-75-0/nefurthiazole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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