| Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: Detection by association analysis of stone-related gene expression and microstructural observation. | |
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MedLine Citation:
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PMID: 20577968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mice have a strong ability to eliminate renal calcium oxalate crystals, and our previous examination indicated a susceptibility in which monocyte-macrophage interaction could participate in the phenomenon. To clarify the macrophage-related factors playing roles in the prevention of crystal formation in mouse kidneys, morphologic and expression studies based on microarray pathway analysis were performed. Eight-week-old male C57BL/6N mice were administered 80 mg/kg of glyoxylate by daily intraabdominal injection for 15 days, and the kidneys were extracted every 3 days for DNA microarray analysis. Based on the raw data of microarray analysis, pathway analyses of inflammatory response demonstrated macrophage activation through the increased expression of chemokine (C-X-C) ligand 1, fibronectin 1, and major histocompatability (MHC) class II. Association analysis of related gene expression values by quantitative reverse transcription polymerase chain reaction (RT-PCR) indicated the high association of chemokine (C-C) ligand 2, CD44, colony-stimulating factor 1, fibronectin 1, matrix gla protein, secreted phosphoprotein 1, and transforming growth factor β1 (TGF-β1) with the amount of both renal crystals and F4/80, a macrophage marker. Immunohistochemically, interstitial macrophages increased during the experimental course, and CD44 and MHC class II were upregulated around crystal-formation sites. Ultrastructural observation of renal macrophages by transmission electron microscopy indicated interstitial macrophage migration with the phagocytosis of crystals. In conclusion, increased expression of inflammation-related genes of renal tubular cells induced by crystal formation and deposition could induce monocyte-macrophage migration and phagocytosis via the interaction of CD44 with osteopontin and fibronectin. Such crystal-removing ability of macrophages through phagocytosis and digestion might become a new target for the prevention of stone formation. |
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Authors:
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Atsushi Okada; Takahiro Yasui; Yasuhiro Fujii; Kazuhiro Niimi; Shuzo Hamamoto; Masahito Hirose; Yoshiyuki Kojima; Yasunori Itoh; Keiichi Tozawa; Yutaro Hayashi; Kenjiro Kohri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-23 |
Journal Detail:
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Title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research Volume: 25 ISSN: 1523-4681 ISO Abbreviation: J. Bone Miner. Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-03-02 Revised Date: 2011-04-28 |
Medline Journal Info:
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Nlm Unique ID: 8610640 Medline TA: J Bone Miner Res Country: United States |
Other Details:
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Languages: eng Pagination: 2701-11 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 American Society for Bone and Mineral Research. |
Affiliation:
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Department of Nephro-urology, Nagoya City University Graduate School of Medical Sciences, Nagoya City, Aichi, Japan. a-okada@med.nagoya-cu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium Oxalate / metabolism* Cell Movement* Gene Expression Regulation Genetic Association Studies Glyoxylates Inflammation / complications, genetics* Kidney / metabolism, pathology*, ultrastructure Kidney Calculi / complications, genetics*, pathology Macrophages / metabolism, pathology*, ultrastructure Male Mice Mice, Inbred C57BL Monocytes / metabolism, pathology Oligonucleotide Array Sequence Analysis Phagocytosis* |
| Chemical | |
Reg. No./Substance:
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0/Glyoxylates; 25454-23-3/Calcium Oxalate; 298-12-4/glyoxylic acid |
| Comments/Corrections | |
Erratum In:
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J Bone Miner Res. 2011 Feb;26(2):439 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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