Document Detail


Renal handling and effects of [3H]digoxin and interactions with quinidine in the avian kidney.
MedLine Citation:
PMID:  3014819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Renal handling and effects of tritium digoxin and interactions with quinidine in the avian kidney were studied using a modified Sperber technique. Results showed that tritium digoxin was extracted at the peritubular side of the nephron in a process competitively inhibited by increasing amounts of unlabelled digoxin. Light microscope autoradiography showed distinct concentrations of silver grains only over distal tubules in the injected kidney. Inhibition of the proximal tubular transport systems for organic anions and cations, respectively, did not change extraction. Addition of quinidine to the injection solution up to an estimated concentration of 1.4 X 10(-5) M in systemic blood significantly lowered 1 min peritubular extraction of tritium digoxin. However, when the amount of quinidine was further increased, extraction of tritium digoxin augmented significantly. Tritium recovery in urine after renal portal bolus injection of tritiated and unlabelled digoxin already showed a distinct ipsilateral peak 2 min after injection with an equally distinct peak of ipsilateral sodium excretion appearing 1 min later. When quinidine was added to the bolus ipsilateral tritium recovery in urine (0-7 min) was halved, with the true tubular excretion fraction (TTEF) lowered by two-thirds, but without changes in the magnitude of ipsilateral natriuresis. Contralateral natriuresis increased more than four-fold with quinidine in the bolus in spite of unchanged tritium recovery in the urine. Thus, our results show tritium digoxin to be extracted from peritubular blood through a specific process probably localized to the distal nephron of the avian kidney.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
A Milton; B Odlind; L Dencker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  127     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-08-08     Completed Date:  1986-08-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  9-16     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Biological Transport, Active / drug effects
Digoxin / administration & dosage,  metabolism*
Drug Interactions
Female
Inulin / administration & dosage
Kidney / metabolism*
Kidney Tubules / metabolism
Natriuresis / drug effects
Poultry
Quinidine / administration & dosage,  metabolism*
Sodium-Potassium-Exchanging ATPase / metabolism
Chemical
Reg. No./Substance:
20830-75-5/Digoxin; 56-54-2/Quinidine; 9005-80-5/Inulin; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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