| Renal cells activate the platelet receptor CLEC-2 through podoplanin. | |
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MedLine Citation:
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PMID: 18215137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have recently shown that the C-type lectin-like receptor, CLEC-2, is expressed on platelets and that it mediates powerful platelet aggregation by the snake venom toxin rhodocytin. In addition, we have provided indirect evidence for an endogenous ligand for CLEC-2 in renal cells expressing HIV-1. This putative ligand facilitates transmission of HIV through its incorporation into the viral envelope and binding to CLEC-2 on platelets. The aim of the present study was to identify the ligand on these cells which binds to CLEC-2 on platelets. Recombinant CLEC-2 exhibits specific binding to HEK-293T (human embryonic kidney) cells in which the HIV can be grown. Furthermore, HEK-293T cells activate both platelets and CLEC-2-transfected DT-40 B-cells. The transmembrane protein podoplanin was identified on HEK-293T cells and was demonstrated to mediate both binding of HEK-293T cells to CLEC-2 and HEK-293T cell activation of CLEC-2-transfected DT-40 B-cells. Podoplanin is expressed on renal cells (podocytes). Furthermore, a direct interaction between CLEC-2 and podoplanin was confirmed using surface plasmon resonance and was shown to be independent of glycosylation of CLEC-2. The interaction has an affinity of 24.5+/-3.7 microM. The present study identifies podoplanin as a ligand for CLEC-2 on renal cells. |
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Authors:
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Charita M Christou; Andrew C Pearce; Aleksandra A Watson; Anita R Mistry; Alice Y Pollitt; Angharad E Fenton-May; Louise A Johnson; David G Jackson; Steve P Watson; Chris A O'Callaghan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 411 ISSN: 1470-8728 ISO Abbreviation: Biochem. J. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-12 Completed Date: 2008-04-15 Revised Date: 2013-04-29 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Henry Wellcome Building for Molecular Physiology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cloning, Molecular Glycosylation Humans Kidney / chemistry, cytology* Lectins, C-Type / metabolism* Ligands Membrane Glycoproteins / analysis, metabolism* Podocytes / chemistry Protein Binding Surface Plasmon Resonance Transfection |
| Grant Support | |
ID/Acronym/Agency:
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G116/165//Medical Research Council; G116/165(64081)//Medical Research Council; PG/07/064/23293//British Heart Foundation; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/CLEC2B protein, human; 0/Lectins, C-Type; 0/Ligands; 0/Membrane Glycoproteins; 0/PDPN protein, human |
| Comments/Corrections | |
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