| Renal allograft rejection--in situ demonstration of cytotoxic intratubular cells. | |
| | |
MedLine Citation:
|
PMID: 8633386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A nonisotopic in situ hybridization method to detect perforin mRNA was developed in cytospin preparations of IL-2-stimulated normal human lymphocytes and applied to formalin-fixed acutely rejected renal transplant material. Individual cells expressing perforin mRNA were localized in severely damaged tubular areas, and a number of these cells appeared to be located inside the tubular basement membrane in close association with tubular epithelial cells. Immunoperoxidase staining in acetone-fixed cryostat sections of acutely rejected kidney confirmed that a considerable proportion of infiltrating cells was CD8+; many of these were in an intratubular location. In addition, perforin protein was identified in individual cells in similar locations to perforin mRNA-positive cells. Again, some intratubular cells were identified. Our findings illustrate that these cells can be fully activated with definite cytotoxic potential. Previously we have demonstrated that T lymphocytes proliferate within the tubular compartment during tubulitis, a characteristic condition in acute renal allograft rejection, and that there is associated tubular epithelial cell proliferation. In this study we think that we have further clarified the consequences of invasion of tubules by lymphoid cells. Our in situ hybridization method in rapid and convenient and may be applied to archival material. |
| | |
Authors:
|
H Robertson; J Wheeler; J A Kirby; A R Morley |
Related Documents
:
|
12012386 - Polygonal epithelial cells in glomerular cell culture: podocyte or parietal epithelial ... 8082126 - A comparative study of the glomerular peripolar cell and the renin-secreting cell in tw... 2345426 - Tissue factor production by cultured rat mesangial cells. stimulation by tnf alpha and ... 2019796 - Effect of 1,25-dihydroxyvitamin d3 on mesangial cell proliferation. 20008336 - The dazzle in germ cell differentiation. 18041636 - Transfection of primary human nasal epithelial cells using a biodegradable poly (ester ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Transplantation Volume: 61 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 1996 May |
Date Detail:
|
Created Date: 1996-07-02 Completed Date: 1996-07-02 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0132144 Medline TA: Transplantation Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1546-9 Citation Subset: IM; X |
Affiliation:
|
Department of Microbiology, The Medical School, University of Newcastle upon Tyne, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute Disease Base Sequence CD8-Positive T-Lymphocytes / immunology* Cytotoxicity, Immunologic DNA Primers / chemistry Graft Rejection / diagnosis* Humans Immunity, Cellular In Situ Hybridization Kidney Transplantation / immunology* Kidney Tubules / immunology Membrane Glycoproteins / genetics Molecular Sequence Data Perforin Pore Forming Cytotoxic Proteins RNA, Messenger / genetics |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/Membrane Glycoproteins; 0/Pore Forming Cytotoxic Proteins; 0/RNA, Messenger; 126465-35-8/Perforin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The use of magnetic beads coated with soluble HLA class I or class II proteins in antibody screening...
Next Document: Successful renal transplantation from two donors with methanol intoxication.