Document Detail

Renal allograft rejection--in situ demonstration of cytotoxic intratubular cells.
MedLine Citation:
PMID:  8633386     Owner:  NLM     Status:  MEDLINE    
A nonisotopic in situ hybridization method to detect perforin mRNA was developed in cytospin preparations of IL-2-stimulated normal human lymphocytes and applied to formalin-fixed acutely rejected renal transplant material. Individual cells expressing perforin mRNA were localized in severely damaged tubular areas, and a number of these cells appeared to be located inside the tubular basement membrane in close association with tubular epithelial cells. Immunoperoxidase staining in acetone-fixed cryostat sections of acutely rejected kidney confirmed that a considerable proportion of infiltrating cells was CD8+; many of these were in an intratubular location. In addition, perforin protein was identified in individual cells in similar locations to perforin mRNA-positive cells. Again, some intratubular cells were identified. Our findings illustrate that these cells can be fully activated with definite cytotoxic potential. Previously we have demonstrated that T lymphocytes proliferate within the tubular compartment during tubulitis, a characteristic condition in acute renal allograft rejection, and that there is associated tubular epithelial cell proliferation. In this study we think that we have further clarified the consequences of invasion of tubules by lymphoid cells. Our in situ hybridization method in rapid and convenient and may be applied to archival material.
H Robertson; J Wheeler; J A Kirby; A R Morley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation     Volume:  61     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-02     Completed Date:  1996-07-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1546-9     Citation Subset:  IM; X    
Department of Microbiology, The Medical School, University of Newcastle upon Tyne, United Kingdom.
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MeSH Terms
Acute Disease
Base Sequence
CD8-Positive T-Lymphocytes / immunology*
Cytotoxicity, Immunologic
DNA Primers / chemistry
Graft Rejection / diagnosis*
Immunity, Cellular
In Situ Hybridization
Kidney Transplantation / immunology*
Kidney Tubules / immunology
Membrane Glycoproteins / genetics
Molecular Sequence Data
Pore Forming Cytotoxic Proteins
RNA, Messenger / genetics
Reg. No./Substance:
0/DNA Primers; 0/Membrane Glycoproteins; 0/Pore Forming Cytotoxic Proteins; 0/RNA, Messenger; 126465-35-8/Perforin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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