| Renal nitric oxide production in rat pregnancy: role of constitutive nitric oxide synthases. | |
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MedLine Citation:
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PMID: 20630934 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Functional studies show that increased renal nitric oxide (NO) mediates the renal vasodilation and increased glomerular filtration rate that occur during normal pregnancy. We investigated whether changes in the constitutive NO synthases (NOS), endothelial (eNOS) and neuronal (nNOS), were associated with the increased renal NO production in normal midterm pregnancy in the rat. In kidneys from midterm pregnant (MP: 11-13 days gestation), late-term pregnant (LP: 18-20 days gestation), and similarly aged virgin (V) rats, transcript and protein abundance for eNOS and the nNOSα and nNOSβ splice variants, as well as the rate of L-arginine-to-L-citrulline conversion, were determined as a measure of NOS activity. At MP, renal cortical abundance of the total eNOS protein and phosphorylated (Ser(1177)) eNOS was reduced, and L-arginine-to-L-citrulline conversion in the cortical membrane fraction was decreased; these declines were also seen in LP. There were no changes in the eNOS transcript. In contrast, L-arginine-to-L-citrulline conversion in the soluble fraction of renal cortex increased at MP and then declined at LP. This MP increase was ablated by S-methylthiocitrulline, a nNOS inhibitor. Using Western blotting, we did not detect a change in the protein abundance or transcript of the 160-kDa nNOSα, but protein abundance and transcript of the nNOSβ were increased at MP in cortex. Collectively, these studies suggest that the soluble nNOSβ is responsible for the increased renal cortical NO production during pregnancy. |
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Authors:
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Cheryl A Smith; Beth Santymire; Aaron Erdely; Vasuki Venkat; György Losonczy; Chris Baylis |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-14 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: 299 ISSN: 1522-1466 ISO Abbreviation: Am. J. Physiol. Renal Physiol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2010-10-26 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: United States |
Other Details:
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Languages: eng Pagination: F830-6 Citation Subset: IM |
Affiliation:
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Department of Physiology and Functional Genomics, University of Florida, Gainesville, Florida, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Glomerular Filtration Rate / physiology Kidney Cortex / blood supply, metabolism* Nitric Oxide / metabolism* Nitric Oxide Synthase / metabolism* Nitric Oxide Synthase Type I / metabolism Nitric Oxide Synthase Type III / metabolism Pregnancy Pregnancy, Animal / metabolism* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Regional Blood Flow / physiology Vasodilation / physiology |
| Grant Support | |
ID/Acronym/Agency:
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DK-56843/DK/NIDDK NIH HHS; HL-31933/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nitric Oxide Synthase Type III |
| Comments/Corrections | |
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