| Removal of the side-chain glucose groups from schizophyllan improves the thermal stability of the polycytidylic acid complexes under the physiological conditions. | |
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MedLine Citation:
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PMID: 15457435 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thermal stabilization of the complex between polycytidylic acid [poly(C)] and the modified schizophyllan (SPG) whose hydrophilic side-chain glucose groups are selectively removed utilizing mild Smith-degradation has been investigated. With the decrease in the side-chain glucose groups of schizophyllan, the complex with poly(C) can be considerably stabilized compared with unmodified SPG; for example, the T(m) value after the removal of the side-chain glucose groups from 33.3 (unmodified) to 1.0 is enhanced by 14 degrees C. In addition, the thermal stabilization effect is even operative under the physiological conditions ([NaCl] = 0.15 mol dm(-3)). This effect is exerted owing to the construction of the hydrophobic atmosphere around the complex. Although schizophyllan lost the side-chain glucose groups, it still kept the protection effect of the bound poly(C) chain against RNaseA-mediated hydrolysis as observed for unmodified schizophyllan. The assessment of the cytotoxicity for A375:human malignant melanoma, and HL60:human promyelocytic leukemia revealed that the modified schizophyllan scarcely increases the cytotoxicity. These results indicate that the present modification for schizophyllan is of great significance in a viewpoint to develop the practical gene carriers operative even under the physiological conditions. |
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Authors:
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Kazuya Koumoto; Ryouji Karinaga; Masami Mizu; Takahisa Anada; Kazuo Sakurai; Toyoki Kunitake; Seiji Shinkai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biopolymers Volume: 75 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-03 Completed Date: 2005-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 403-11 Citation Subset: IM |
Copyright Information:
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Copyright 2004 Wiley Periodicals, Inc. |
Affiliation:
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Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Survival Drug Stability Glucose / chemistry* Humans Hydrolysis Poly C / chemistry* Ribonuclease, Pancreatic / chemistry Sizofiran / chemistry*, toxicity Temperature |
| Chemical | |
Reg. No./Substance:
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30811-80-4/Poly C; 50-99-7/Glucose; 9050-67-3/Sizofiran; EC 3.1.27.5/Ribonuclease, Pancreatic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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