Document Detail


Removal of rat dendritic cells from single cell suspensions by passage through columns of Sephadex G-10.
MedLine Citation:
PMID:  6209339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat T lymphocytes require dendritic cells as accessory cells in order to respond to the mitogen sodium periodate. Passage of a lymph node cell suspension through a column of Sephadex G-10 reduced the mitogenic response by greater than 90%, despite a cell recovery of 75%. An even greater reduction in the response occurred after a second passage over Sephadex G-10; addition of purified dendritic cells restored the response. Lymph node cell suspensions and preparations enriched in dendritic cells were nearly depleted of these cells by passage over Sephadex G-10. After passage of lymph node cells over Sephadex G-10, a limited number of retained cells could be recovered; these included both dendritic cells and macrophages. Enumeration of macrophages in the various passed and retained fractions by non-specific esterase staining of smears confirmed that macrophages were effectively removed from lymph node cell suspensions by repeated passage over Sephadex G-10. Cell preparations that pass through Sephadex G-10 are therefore depleted of both dendritic cells and macrophages.
Authors:
W E Bowers; M R Berkowitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  74     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1985-01-23     Completed Date:  1985-01-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  317-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Cells, Cultured
Chromatography, Gel / methods
DNA Replication
Dextrans
Female
Lymph Nodes / cytology*
Lymphocyte Activation
Male
Rats
Rats, Inbred Lew
T-Lymphocytes / cytology*,  immunology
Grant Support
ID/Acronym/Agency:
AI-17887/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
9004-54-0/Dextrans; 9014-76-0/sephadex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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