| Removal of rat dendritic cells from single cell suspensions by passage through columns of Sephadex G-10. | |
| | |
MedLine Citation:
|
PMID: 6209339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rat T lymphocytes require dendritic cells as accessory cells in order to respond to the mitogen sodium periodate. Passage of a lymph node cell suspension through a column of Sephadex G-10 reduced the mitogenic response by greater than 90%, despite a cell recovery of 75%. An even greater reduction in the response occurred after a second passage over Sephadex G-10; addition of purified dendritic cells restored the response. Lymph node cell suspensions and preparations enriched in dendritic cells were nearly depleted of these cells by passage over Sephadex G-10. After passage of lymph node cells over Sephadex G-10, a limited number of retained cells could be recovered; these included both dendritic cells and macrophages. Enumeration of macrophages in the various passed and retained fractions by non-specific esterase staining of smears confirmed that macrophages were effectively removed from lymph node cell suspensions by repeated passage over Sephadex G-10. Cell preparations that pass through Sephadex G-10 are therefore depleted of both dendritic cells and macrophages. |
| | |
Authors:
|
W E Bowers; M R Berkowitz |
Related Documents
:
|
3501579 - Stereospecific inhibition of human epidermal cell interleukin-1 secretion and hla-dr ex... 9740509 - S-100 protein-positive dendritic cells in follicular lesions of the thyroid. 9553909 - Langerhans cells in benign, premalignant and malignant skin lesions of renal transplant... 3706669 - Quantitative studies on nonlymphoid mononuclear cell subpopulations in cutaneous infilt... 11069529 - Huriez syndrome: case report with a detailed analysis of skin dendritic cells. 7236519 - Cutaneous ultrastructural diagnosis of ceroid-lipofuscinosis. 17561979 - Role of the endothelin axis in the proliferation of human thyroid cancer cells. 22707119 - Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on... 7300429 - Purification of bovine adrenal chromaffin cells by differential plating. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of immunological methods Volume: 74 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1984 Nov |
Date Detail:
|
Created Date: 1985-01-23 Completed Date: 1985-01-23 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 317-25 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Separation / methods* Cells, Cultured Chromatography, Gel / methods DNA Replication Dextrans Female Lymph Nodes / cytology* Lymphocyte Activation Male Rats Rats, Inbred Lew T-Lymphocytes / cytology*, immunology |
| Grant Support | |
ID/Acronym/Agency:
|
AI-17887/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
9004-54-0/Dextrans; 9014-76-0/sephadex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies.
Next Document: Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with imm...