| Removal of the glycosylation of prion protein provokes apoptosis in SF126. | |
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MedLine Citation:
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PMID: 17927898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although the function of cellular prion protein (PrPc) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis. |
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Authors:
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Lan Chen; Yang Yang; Jun Han; Bao-Yun Zhang; Lin Zhao; Kai Nie; Xiao-Fan Wang; Feng Li; Chen Gao; Xiao-Ping Dong; Cai-Min Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry and molecular biology Volume: 40 ISSN: 1225-8687 ISO Abbreviation: J. Biochem. Mol. Biol. Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-10-11 Completed Date: 2008-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9702084 Medline TA: J Biochem Mol Biol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 662-9 Citation Subset: IM |
Affiliation:
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National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Dong Dan San Tiao 5, Beijing 100005, Peopleos Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / genetics, physiology* Blotting, Western Brain / metabolism*, pathology Cell Line, Tumor Cricetinae Flow Cytometry Fluorescent Antibody Technique Glycosylation Humans Membrane Potential, Mitochondrial Mutation Nucleotides / metabolism Plasmids / genetics PrPSc Proteins / metabolism Prions / genetics, metabolism* Proto-Oncogene Proteins c-bcl-2 / metabolism Transfection bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Nucleotides; 0/PrPSc Proteins; 0/Prions; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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