Document Detail


Removal of common Fusarium toxins in vitro by strains of Lactobacillus and Propionibacterium.
MedLine Citation:
PMID:  12113664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was conducted to examine the ability of selected strains of Lactobacillus and Propionibacterium to remove common Fusarium toxins, trichothecenes, from liquid media. The trichothecenes studied were deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), nivalenol (NIV), fusarenon (FX), diacetoxyscirpenol (DAS), T-2 toxin (T-2) and HT-2 toxin (HT-2). The Lactobacillus rhamnosus strain GG (LGG), Lactobacillus rhamnosus strain LC-705 (LC-705) and Propionibacterium freudenreichii ssp. shermanii JS (PJS) were incubated in PBS buffer containing 20 microg toxin ml(-1) for 1h at 37 degrees C, and after centrifugation the concentration of the toxins was measured in the supernatant fraction. Both viable and heat-killed forms of LGG and PJS were more efficient than LC-705 in removing the toxins from the liquid media. LGG and PJS removed four of the seven tested toxins (the removal varying from 18 to 93%) and LC-705 two toxins (10-64%). Of the toxins, 3-AcDON was not removed by any of the bacteria; HT-2 was removed by the non-viable LGG and also slightly by non-viable LC-705; DAS was removed by all three bacteria tested. Binding is postulated as the possible mechanism of the removal, since no difference was observed between the ability of viable and heat-killed bacteria in removing the trichothecenes, and no degradation products of the toxins were detected by gas chromatography (GC)-mass spectrometry (MS) analysis. It is concluded that significant differences exist in the ability of the bacteria to bind trichothecenes in vitro.
Authors:
H S El-Nezami; A Chrevatidis; S Auriola; S Salminen; H Mykkänen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Food additives and contaminants     Volume:  19     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-12     Completed Date:  2002-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  680-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Nutrition, Food and Health Research Center, University of Kuopio, PO Box FIN-1627, 70211, Kuopio, Finland. Hani.El-nezami@uku.fi
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MeSH Terms
Descriptor/Qualifier:
Decontamination / methods
Fusarium / metabolism*
Gas Chromatography-Mass Spectrometry / methods
Lactobacillus / metabolism*
Propionibacterium / metabolism*
Trichothecenes / metabolism*
Chemical
Reg. No./Substance:
0/Trichothecenes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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