Document Detail

Removal of polysialic acid triggers dispersion of subventricularly derived neuroblasts into surrounding CNS tissues.
MedLine Citation:
PMID:  20237270     Owner:  NLM     Status:  MEDLINE    
Cells generated in the subventricular zone give rise to neuroblasts that migrate to the olfactory bulb (OB) along the rostral migratory stream (RMS). The polysialylated form of neural cell adhesion molecule (PSA-NCAM) is expressed by these cells, and has been shown to both promote their migration and suppress differentiation induced by NCAM. In the present study, enzymatic removal of PSA from these neuroblasts using PSA-specific endoneuraminidase has been found not only to disrupt the tangential migration and cellular organization of the RMS, but also to cause a massive dispersion of BrdU (5-bromo-2'-deoxyuridine)-labeled neuroblasts into surrounding brain regions, including cortex and striatum. These dispersed cells are capable of differentiation, some into mature neurons, and could potentially be of value in the repair of CNS injury. Although the removal of PSA by genetic deletion of NCAM also results in a smaller OB and a swollen RMS, the cells do not escape the RMS in large numbers. These findings suggest that the presence of NCAM without PSA plays a role in the dispersion process, possibly by inducing a new pattern of migration associated with NCAM-dependent differentiation.
Daniela Battista; Urs Rutishauser
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  30     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3995-4003     Citation Subset:  IM    
Laboratory of Cellular and Developmental Neuroscience, Department of Cell Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Brain / cytology,  metabolism,  physiology
Bromodeoxyuridine / diagnostic use
Cell Differentiation / physiology
Cell Movement / physiology*
Cerebral Ventricles / cytology*,  metabolism,  physiology*
Glycoside Hydrolases / pharmacology
Mice, Inbred BALB C
Mice, Knockout
Neural Cell Adhesion Molecules / biosynthesis,  physiology
Neurogenesis / physiology
Neurons / cytology*,  metabolism,  physiology*
Olfactory Bulb / cytology,  metabolism,  physiology
Sialic Acids / deficiency*
Stem Cells / cytology*,  metabolism,  physiology*
Reg. No./Substance:
0/Neural Cell Adhesion Molecules; 0/Sialic Acids; 0/polysialic acid; 59-14-3/Bromodeoxyuridine; EC 3.2.1.-/Glycoside Hydrolases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  RET signaling is required for survival and normal function of nonpeptidergic nociceptors.
Next Document:  Dyrk1A overexpression inhibits proliferation and induces premature neuronal differentiation of neura...