Document Detail

Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.
MedLine Citation:
PMID:  19687136     Owner:  NLM     Status:  MEDLINE    
Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.
Alice Witsell; Daniel P Kane; Sarah Rubin; Mitch McVey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-08-17
Journal Detail:
Title:  Genetics     Volume:  183     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-04-15     Revised Date:  2013-02-11    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1187-93     Citation Subset:  IM    
Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
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MeSH Terms
DNA Breaks, Double-Stranded
DNA Helicases / genetics*
DNA Repair
DNA Transposable Elements / genetics*
Drosophila melanogaster / enzymology,  genetics*
Fertility / genetics
Gene Deletion
Genome, Insect / genetics
Mutagenesis, Insertional / methods*
Reg. No./Substance:
0/DNA Transposable Elements; EC 3.6.1.-/DNA Helicases; EC protein, Drosophila

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