Document Detail


Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila.
MedLine Citation:
PMID:  19687136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements.
Authors:
Alice Witsell; Daniel P Kane; Sarah Rubin; Mitch McVey
Related Documents :
16814186 - Blepharophimosis, ptosis, and epicanthus inversus syndrome: clinical and molecular anal...
17330256 - A splice site mutation confirms the role of lpin2 in majeed syndrome.
16541386 - Trafficking-competent and trafficking-defective kcnj2 mutations in andersen syndrome.
23283016 - Pancake syndrome (oral mite anaphylaxis).
12210296 - Behavioral phenotypes in four mental retardation syndromes: fetal alcohol syndrome, pra...
19196886 - Phosphodiesterase 5 inhibition improves beta-cell function in metabolic syndrome.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-08-17
Journal Detail:
Title:  Genetics     Volume:  183     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-04-15     Revised Date:  2013-02-11    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1187-93     Citation Subset:  IM    
Affiliation:
Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
DNA Breaks, Double-Stranded
DNA Helicases / genetics*
DNA Repair
DNA Transposable Elements / genetics*
Drosophila melanogaster / enzymology,  genetics*
Female
Fertility / genetics
Gene Deletion
Genome, Insect / genetics
Male
Mutagenesis, Insertional / methods*
Mutation*
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; EC 3.6.1.-/DNA Helicases; EC 3.6.4.12/blm protein, Drosophila
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Incidence and correlates of 'catastrophic' maternal health care expenditure in India.
Next Document:  Suppression of mitochondrial DNA instability of autosomal dominant forms of progressive external oph...