| Removal of the bloom syndrome DNA helicase extends the utility of imprecise transposon excision for making null mutations in Drosophila. | |
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MedLine Citation:
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PMID: 19687136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements. |
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Authors:
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Alice Witsell; Daniel P Kane; Sarah Rubin; Mitch McVey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-08-17 |
Journal Detail:
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Title: Genetics Volume: 183 ISSN: 1943-2631 ISO Abbreviation: Genetics Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-25 Completed Date: 2010-04-15 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 1187-93 Citation Subset: IM |
Affiliation:
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Department of Biology, Tufts University, Medford, Massachusetts 02155, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA Breaks, Double-Stranded DNA Helicases / genetics* DNA Repair DNA Transposable Elements / genetics* Drosophila melanogaster / enzymology, genetics* Female Fertility / genetics Gene Deletion Genome, Insect / genetics Male Mutagenesis, Insertional / methods* Mutation* |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; EC 2.7.7.-/blm protein, Drosophila; EC 3.6.1.-/DNA Helicases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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