Document Detail


Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environments.
MedLine Citation:
PMID:  11987307     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.
Authors:
Michael S Manak; Anna-Lisa Paul; Paul C Sehnke; Robert J Ferl
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Life support & biosphere science : international journal of earth space     Volume:  8     ISSN:  1069-9422     ISO Abbreviation:  Life Support Biosph Sci     Publication Date:  2002  
Date Detail:
Created Date:  2002-05-02     Completed Date:  2002-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9431217     Medline TA:  Life Support Biosph Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-91     Citation Subset:  S    
Affiliation:
Plant Molecular and Cellular Biology, Horticulture Sciences Department, University of Florida, Gainesville, FL 32611-0690, USA.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Dehydrogenase / genetics
Arabidopsis / genetics*,  physiology
Biosensing Techniques*
Extraterrestrial Environment*
Gene Expression Regulation, Plant*
Genes, Reporter
Green Fluorescent Proteins
Image Processing, Computer-Assisted*
Luminescent Proteins / genetics
Plant Physiological Phenomena
Plants, Genetically Modified / genetics*,  physiology
Promoter Regions, Genetic
Telemetry
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; EC 1.1.1.1/Alcohol Dehydrogenase
Investigator
Investigator/Affiliation:
R J Ferl / U FL, Gainesville

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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