Document Detail

Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environments.
MedLine Citation:
PMID:  11987307     Owner:  NASA     Status:  MEDLINE    
Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.
Michael S Manak; Anna-Lisa Paul; Paul C Sehnke; Robert J Ferl
Related Documents :
17657537 - Plasmid vectors for testing in vivo promoter activities in corynebacterium glutamicum a...
24657757 - Nitrogen removal pathway of anaerobic ammonium oxidation in on-site aged refuse bioreac...
20858267 - Spindle assembly checkpoint genes reveal distinct as well as overlapping expression tha...
12639307 - Improved herpes simplex virus type 1 amplicon vectors for proportional coexpression of ...
25422937 - Antibiotic genes spread far and wide.
15485927 - Functional characterization of a trypanosoma brucei tata-binding protein-related factor...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Life support & biosphere science : international journal of earth space     Volume:  8     ISSN:  1069-9422     ISO Abbreviation:  Life Support Biosph Sci     Publication Date:  2002  
Date Detail:
Created Date:  2002-05-02     Completed Date:  2002-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9431217     Medline TA:  Life Support Biosph Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-91     Citation Subset:  S    
Plant Molecular and Cellular Biology, Horticulture Sciences Department, University of Florida, Gainesville, FL 32611-0690, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alcohol Dehydrogenase / genetics
Arabidopsis / genetics*,  physiology
Biosensing Techniques*
Extraterrestrial Environment*
Gene Expression Regulation, Plant*
Genes, Reporter
Green Fluorescent Proteins
Image Processing, Computer-Assisted*
Luminescent Proteins / genetics
Plant Physiological Phenomena
Plants, Genetically Modified / genetics*,  physiology
Promoter Regions, Genetic
Reg. No./Substance:
0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; EC Dehydrogenase
R J Ferl / U FL, Gainesville

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Human factor observations of the Biosphere 2, 1991-1993, closed life support human experiment and it...
Next Document:  Plant adaptation to low atmospheric pressures: potential molecular responses.