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Remote ischemic preconditioning preserves Connexin 43 phosphorylation in the rat heart in vivo.
MedLine Citation:
PMID:  25159820     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: Remote ischemic preconditioning (RIPC) protects the heart from ischemia and reperfusion (I/R) injury. The underlying molecular mechanisms are unclear. It has been demonstrated that Connexin 43 (Cx43) is critically involved in cardioprotective interventions including classical ischemic preconditioning. In the present study we investigated the influence of RIPC on the expression patterns of Cx43 after I/R in the rat heart in vivo.
METHODS: Male Wistar rats were subjected to 35 min regional myocardial ischemia followed by 2 h reperfusion with or without 4 cycles of 5 minutes bilateral hind limb ischemia and reperfusion (RIPC), to RIPC without ischemia or underwent no intervention (Sham). Infarct size was measured by TTC staining. The myocardium was divided into area at risk (AAR) and area not at risk (non AAR). Expression of Cx43-mRNA and protein was analyzed by qPCR and Western Blot analysis, respectively. Localization of Cx43 was visualized by confocal immunofluorescence staining.
RESULTS: RIPC reduced the infarct size (I/R: 73 ± 5% vs.
RIPC I/R: 34 ± 14%, p < 0.05). Expression of Cx43 mRNA did not differ between groups. I/R caused a strong decrease of relative Cx43 protein expression in the AAR that was partly abolished by RIPC. Furthermore, RIPC decreased the level of ischemia-induced dephosphorylation of Cx43. Confocal immunofluorescence staining showed that I/R caused a loss of the Cx43 signal at the intercalated discs, while the Cx43 signal at the intercalated discs was partly sustained after RIPC.
CONCLUSION: Preservation of Cx43 protein expression and phosphorylation after RIPC might protect the rat heart in vivo.
Authors:
Timo Brandenburger; Ragnar Huhn; Andreas Galas; Benedikt H Pannen; Verena Keitel; Franziska Barthel; Inge Bauer; André Heinen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2014-08-27
Journal Detail:
Title:  Journal of translational medicine     Volume:  12     ISSN:  1479-5876     ISO Abbreviation:  J Transl Med     Publication Date:  2014  
Date Detail:
Created Date:  2014-09-30     Completed Date:  -     Revised Date:  2014-12-06    
Medline Journal Info:
Nlm Unique ID:  101190741     Medline TA:  J Transl Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  228     Citation Subset:  IM    
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