Document Detail


Remote focal scanning optical projection tomography with an electrically tunable lens.
MedLine Citation:
PMID:  25360356     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.
Authors:
Lingling Chen; Sunil Kumar; Douglas Kelly; Natalie Andrews; Margaret J Dallman; Paul M W French; James McGinty
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Publication Detail:
Type:  Journal Article     Date:  2014-09-02
Journal Detail:
Title:  Biomedical optics express     Volume:  5     ISSN:  2156-7085     ISO Abbreviation:  Biomed Opt Express     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-31     Completed Date:  2014-10-31     Revised Date:  2014-11-03    
Medline Journal Info:
Nlm Unique ID:  101540630     Medline TA:  Biomed Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3367-75     Citation Subset:  -    
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