Document Detail


Remote control of myosin and kinesin motors using light-activated gearshifting.
MedLine Citation:
PMID:  25086603     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cytoskeletal motors perform critical force generation and transport functions in eukaryotic cells. Engineered modifications of motor function provide direct tests of protein structure-function relationships and potential tools for controlling cellular processes or for harnessing molecular transport in artificial systems. Here, we report the design and characterization of a panel of cytoskeletal motors that reversibly change gears-speed up, slow down or switch directions-when exposed to blue light. Our genetically encoded structural designs incorporate a photoactive protein domain to enable light-dependent conformational changes in an engineered lever arm. Using in vitro motility assays, we demonstrate robust spatiotemporal control over motor function and characterize the kinetics of the optical gearshifting mechanism. We have used a modular approach to create optical gearshifting motors for both actin-based and microtubule-based transport.
Authors:
Muneaki Nakamura; Lu Chen; Stuart C Howes; Tony D Schindler; Eva Nogales; Zev Bryant
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-03
Journal Detail:
Title:  Nature nanotechnology     Volume:  -     ISSN:  1748-3395     ISO Abbreviation:  Nat Nanotechnol     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101283273     Medline TA:  Nat Nanotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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