Document Detail

Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers.
MedLine Citation:
PMID:  22582171     Owner:  NLM     Status:  MEDLINE    
Controlled activation or release of biomolecules is very crucial in various biological applications. Controlling the activity of biomolecules have been attempted by various means and controlling the activity by light has gained popularity in the past decade. The major hurdle in this process is that photoactivable compounds mostly respond to UV radiation and not to visible or near-infrared (NIR) light. The use of UV irradiation is limited by its toxicity and very low tissue penetration power. In this study, we report the exploitation of the potential of NIR-to-UV upconversion nanoparticles (UCNs), which act as nanotransducers to absorb NIR light having high tissue penetration power and negligible phototoxicity and emit UV light locally, for photoactivation of caged compounds and, in particular, used for photo-controlled gene expression. Both activation and knockdown of GFP was performed in both solution and cells, and patterned activation of GFP was achieved successfully by using upconverted UV light produced by NIR-to-UV UCNs. In-depth photoactivation through tissue phantoms and in vivo activation of caged nucleic acids were also accomplished. The success of this methodology has defined a unique level in the field of photo-controlled activation and delivery of molecules.
Muthu Kumara Gnanasammandhan Jayakumar; Niagara Muhammad Idris; Yong Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-10
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-30     Completed Date:  2012-08-24     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8483-8     Citation Subset:  IM    
Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117576.
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MeSH Terms
Cell Line, Tumor
Cell Survival / radiation effects
Comet Assay
DNA / chemistry,  genetics,  metabolism
DNA Damage
Green Fluorescent Proteins / chemistry,  genetics,  metabolism
Hindlimb / metabolism,  radiation effects
Infrared Rays
Mice, Inbred BALB C
Microscopy, Confocal
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Muscle, Skeletal / metabolism,  radiation effects
Nanoparticles / chemistry*,  ultrastructure
Nanotechnology / instrumentation,  methods*
Photochemical Processes / radiation effects
Photochemistry / instrumentation,  methods*
RNA, Small Interfering / chemistry,  genetics,  metabolism
Ultraviolet Rays
Reg. No./Substance:
0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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