| Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats. | |
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MedLine Citation:
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PMID: 17398390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Venous dilatation and wall thickening are part of the maturation of an arteriovenous fistula (AVF). However, the underlying mechanism of AVF remodeling remains unknown. We therefore studied whether matrix remodeling elicited by matrix metalloproteinases (MMPs) may contribute to AVF maturation. METHODS: A femoral AVF model in rats was established by invagination of the distal end of the left femoral artery into the femoral vein after venotomy (fistula group). In the sham group, the left femoral artery was cut, but venous invagination was not performed. Changes in the hemodynamics and the diameter of the iliac vein were studied on days 3, 14, and 28, then the iliac vein was removed and examined for changes in wall thickness and expression of MMP-2 and MMP-9, type 4 tissue inhibitor of metalloproteinases (TIMP-4), and collagen I and III by immunohistochemical staining or Western blotting. RESULTS: Femoral AVF resulted in a sixfold increase in blood flow in the fistula iliac vein and a gradual, but significant, increase in the thickness of the intima and media and marked up-regulation of MMP-2 and MMP-9, down-regulation of TIMP-4, as well as degradation of collagens I and III. The collagen I/III ratio was significantly higher in the 14-day fistula group (1.44 +/- 0.32) than in the sham group (0.82 +/- 0.15) and was even higher in the 28-day fistula group (1.76 +/- 0.21). CONCLUSION: The present results confirmed our hypothesis that a high blood flow rate in the fistula vein affects the expression of MMPs and TIMP-4, resulting in the remodeling or maturation of the AVF. Remodeling is associated with degradation of collagen, with an increase in the collagen I/III ratio. |
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Authors:
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Chih-Yang Chan; Yih-Sharng Chen; Ming-Chieh Ma; Chau-Fong Chen; Chou-Fong Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of vascular surgery : official publication, the Society for Vascular Surgery [and] International Society for Cardiovascular Surgery, North American Chapter Volume: 45 ISSN: 0741-5214 ISO Abbreviation: J. Vasc. Surg. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-02 Completed Date: 2007-05-17 Revised Date: 2007-06-27 |
Medline Journal Info:
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Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: United States |
Other Details:
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Languages: eng Pagination: 804-11 Citation Subset: IM |
Affiliation:
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Department of Surgery, Cardiovascular Division, Far Eastern Memorial Hospital, Panchaio, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteriovenous Shunt, Surgical* Blood Flow Velocity Blood Pressure Blotting, Western Collagen Type I / metabolism Collagen Type III / metabolism Collagenases / biosynthesis* Enzyme Induction Female Femoral Artery / surgery* Femoral Vein / surgery* Fibrillar Collagens / metabolism* Gene Expression Regulation, Enzymologic Hyperplasia Iliac Vein / enzymology*, pathology, physiopathology Immunohistochemistry Matrix Metalloproteinase 2 / biosynthesis Matrix Metalloproteinase 9 / biosynthesis Rats Rats, Wistar Time Factors Tissue Inhibitor of Metalloproteinases / metabolism Tunica Intima / pathology Tunica Media / pathology Up-Regulation Vasodilation |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Collagen Type III; 0/Fibrillar Collagens; 0/Tissue Inhibitor of Metalloproteinases; 0/tissue inhibitor of metalloproteinase-4; EC 3.4.24.-/Collagenases; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
| Comments/Corrections | |
Erratum In:
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J Vasc Surg. 2007 Jun;45(6):1293 Note: Chen, Chou-Fong [corrected to Chen, Chau-Fong] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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