| Remodeling of cardiolipin by phospholipid transacylation. | |
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MedLine Citation:
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PMID: 14551214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mitochondrial cardiolipin (CL) contains unique fatty acid patterns, but it is not known how the characteristic molecular species of CL are formed. We found a novel reaction that transfers acyl groups from phosphatidylcholine or phosphatidylethanolamine to CL in mitochondria of rat liver and human lymphoblasts. Acyl transfer was stimulated by ADP, ATP, and ATP gamma S, but not by other nucleotides. Coenzyme A stimulated the reaction only in the absence of adenine nucleotides. Free fatty acids were not incorporated into CL under the same incubation condition. The transacylation required addition of exogenous CL or monolyso-CL, whereas dilyso-CL was not a substrate. Transacylase activity was decreased in lymphoblasts from patients with Barth syndrome (tafazzin deletion), and this was accompanied by drastic changes in the molecular composition of CL. In rat liver, where linoleic acid was the most abundant residue of CL, only linoleoyl groups were transferred into CL, but not oleoyl or arachidonoyl groups. We demonstrated complete remodeling of tetraoleoyl-CL to tetralinoleoyl-CL in rat liver mitochondria and identified the intermediates linoleoyl-trioleoyl-CL, dilinoleoyl-dioleoyl-CL, and trilinoleoyl-oleoyl-CL by high-performance liquid chromatography. The data suggest that CL is remodeled by acyl specific phospholipid transacylation and that tafazzin is an acyltransferase involved in this mechanism. |
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Authors:
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Yang Xu; Richard I Kelley; Thomas J J Blanck; Michael Schlame |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-10-09 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 278 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-12-15 Completed Date: 2004-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 51380-5 Citation Subset: IM |
Affiliation:
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Department of Anesthesiology, New York University School of Medicine, New York, New York 10016, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acylation Acyltransferases / metabolism* Animals Carbon Radioisotopes Cardiolipins / metabolism* Case-Control Studies Humans Lipid Metabolism, Inborn Errors / metabolism* Lymphocytes / metabolism Mitochondria, Liver / metabolism Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism Proteins / metabolism Rats Transcription Factors* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Cardiolipins; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Proteins; 0/TAZ protein, human; 0/Transcription Factors; EC 2.3.-/Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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