Document Detail


Remnant lipoprotein cholesterol homogenous assay (RemL-C) is closely associated with very-low-density lipoprotein remnants: comparison with the immunoseparation assay.
MedLine Citation:
PMID:  19086452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Remnant-like particle-cholesterol (RLP-C) is recognized as a risk factor for cardiovascular disease. As an alternative to the immunoseparation assay widely used for the measurement of RLP-C, a new remnant lipoprotein-C homogenous assay (RemL-C) is available. In light of its homogeneity as an assay method, we speculated that this homogeneous assay (RemL-C) is closely associated with very-low-density lipoprotein(VLDL) remnant including intermediate-density lipoprotein(IDL). We examined the characteristics of the homogeneous assay for reacting with VLDL remnants. METHODS AND RESULTS: VLDL1, VLDL2, and IDL were separated by ultracentrifugation in the fasting serum of subjects including hypertriglyceridemia and uremic patients usually having higher levels of remnants. While RemL-C and RLP-C were mainly recovered in VLDL1 and both assays were strongly correlated with serum TG and VLDL1, the RemL-C assay was more closely correlated with VLDL2 and IDL levels than the RLP-C assay. RemL-C levels were significantly correlated with IDL-C, whereas RLP-C levels had only borderline associations with IDL-C (r= 0.56 Vs. 0.31). CONCLUSIONS: The remnant lipoprotein cholesterol homogenous assay is more closely associated with VLDL2 and IDL than the immunoseparation assay.
Authors:
Masaharu Nagashima; Yusaku Mori; Ryo Morita; Michiya Takada; Soushou Lee; Takayasu Taira; Kazuhito Miyauchi; Hideyuki Kuwata; Norihiko Kayahara; Tsutomu Hirano
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Rinsho byori. The Japanese journal of clinical pathology     Volume:  56     ISSN:  0047-1860     ISO Abbreviation:  Rinsho Byori     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-02-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984781R     Medline TA:  Rinsho Byori     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  973-9     Citation Subset:  IM    
Affiliation:
Department of Diabetes, Metabolism and Endocrinology, Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8666, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers / blood
Cardiovascular Diseases / etiology
Cholesterol / blood*
Cholesterol, VLDL / blood*
Humans
Hyperlipidemias / blood
Immunoassay / methods*
Lipoproteins / blood*
Middle Aged
Renal Dialysis
Risk Factors
Triglycerides / blood*
Uremia / blood
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cholesterol, VLDL; 0/Lipoproteins; 0/Triglycerides; 0/remnant-like particle cholesterol; 57-88-5/Cholesterol

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