| Remarkable coexistence of multiple cytotypes of the Gymnadenia conopsea aggregate (the fragrant orchid): evidence from flow cytometry. | |
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MedLine Citation:
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PMID: 21059612 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: One of the prerequisites for polyploid research in natural systems is knowledge of the geographical distribution of cytotypes. Here inter- and intrapopulational ploidy diversity was examined in the Gymnadenia conopsea aggregate in central Europe and potential explanations and evolutionary consequences of the observed spatial patterns investigated. METHODS: DAPI flow cytometry supplemented by confirmatory chromosome counts was used to determine ploidy in 3581 samples of the G. conopsea aggregate from 43 populations. The fine-scale spatial pattern of cytotype distribution (intra- and interploidy associations) was analysed with univariate and bivariate K-functions. KEY RESULTS: Gymnadenia tissues undergo a progressively partial endoreplication, which accounts for about 60 % and 75 % of the total genome in G. conopsea and G. densiflora, respectively. Flow cytometric profiles are therefore species-specific and can be used as a marker for rapid and reliable species recognition. Two majority (4x, 8x) and three minority (6x, 10x, 12x) cytotypes were found, often in mixed-ploidy populations (harbouring up to all five different ploidy levels). The scarcity of the minority cytotypes (about 2·7 %) suggests the existence of strong pre- or postzygotic mating barriers. Spatial structure was observed in plots of populations with the highest cytotype variation, including clumping of individuals of the same ploidy and negative association between tetra- and octoploids. CONCLUSIONS: The remarkable ploidy coexistence in the G. conopsea aggregate has reshaped our perception of intrapopulational ploidy diversity under natural conditions. This system offers unique opportunities for studying processes governing the formation and establishment of polyploids and assessing the evolutionary significance of the various pre- and postzygotic mating barriers that maintain this ploidy mixture. |
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Authors:
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Pavel Trávnícek; Barbora Kubátová; Vladislav Curn; Jana Rauchová; Eva Krajníková; Jana Jersáková; Jan Suda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-07 |
Journal Detail:
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Title: Annals of botany Volume: 107 ISSN: 1095-8290 ISO Abbreviation: Ann. Bot. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-17 Completed Date: 2011-03-24 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 0372347 Medline TA: Ann Bot Country: England |
Other Details:
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Languages: eng Pagination: 77-87 Citation Subset: IM |
Affiliation:
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Institute of Botany, Academy of Sciences of the Czech Republic, CZ-252 43 Průhonice, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Plant Czech Republic DNA, Plant / genetics* Flow Cytometry Genetic Variation* Genome, Plant Orchidaceae / classification, genetics* Polyploidy Slovakia Tetraploidy |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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