| Remaining rod activity mediates visual behavior in adult Rpe65-/- mice. | |
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MedLine Citation:
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PMID: 20702833 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: C57/Bl6, Cpfl1(-/-) (cone photoreceptors function loss 1; pure rod function), Gnat1a(-/-) (rod α-transducin; pure cone function), and Rpe65(-/-);Rho(-/-) double-knockout mice were studied to distinguish the respective contributions of the different photoreceptor (PR) systems that enable light perception and mediate a visual reflex in adult Rpe65(-/-) mice, with a simple behavioral procedure. METHODS: Visual function was estimated using a rotating automated optomotor drum covered with vertical black-and-white stripes at spatial frequencies of 0.025 to 0.5 cycles per degree (cyc/deg) in both photopic and scotopic conditions. Mouse strains with different luminances were tested to evaluate the contribution and the light-intensity threshold of each PR system. RESULTS: Stripe rotation elicited head movements in the wild-type (WT) animals in photopic and scotopic conditions, depending on the spatial frequency, whereas the Cpfl1(-/-) mice show a reduced activity in the photopic condition and the Gnat1a(-/-) mice an almost absent response in the scotopic condition. A robust visual response was obtained with Rpe65(-/-) knockout mice at 0.075 and 0.1 cyc/deg in the photopic condition. The residual rod function in the Rpe65(-/-) animals was demonstrated by testing Rpe65(-/-);Rho(-/-) mice that present no response in photopic conditions. CONCLUSIONS: The optomotor test is a simple method of estimating the visual function and evaluating the respective contributions of rod and cone systems. This test was used to demonstrate that in Rpe65(-/-) mice, devoid of functional cones and of detectable 11-cis-retinal protein, the rods mimic cone function in part, by mediating vision in photopic conditions. |
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Authors:
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Maité Cachafeiro; Alexis-Pierre Bemelmans; Kriss Canola; Vérène Pignat; Sylvain Vincent Crippa; Corinne Kostic; Yvan Arsenijevic |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-11 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 51 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-02 Completed Date: 2011-01-13 Revised Date: 2012-05-28 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 6835-42 Citation Subset: IM |
Affiliation:
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Ophthalmology Department, University of Lausanne, Jules-Gonin Eye Hospital, Lausanne, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Behavior, Animal / physiology* Carrier Proteins / genetics* Color Vision / physiology Eye Proteins / genetics* GTP-Binding Protein alpha Subunits / genetics Gene Silencing / physiology* Male Mice Mice, Inbred C57BL Mice, Knockout Motor Activity / physiology Night Vision / physiology Retinal Degeneration / genetics, physiopathology* Retinal Rod Photoreceptor Cells / physiology* Transducin / genetics Vision, Ocular / physiology* Visual Perception / physiology* rho GTP-Binding Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Eye Proteins; 0/GTP-Binding Protein alpha Subunits; 0/Gnat1 protein, mouse; EC 3.1.1.64/retinoid isomerohydrolase; EC 3.6.1.-/Transducin; EC 3.6.5.2/rho GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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