Document Detail


Relocalization of the polypyrimidine tract-binding protein during PKA-induced neurite growth.
MedLine Citation:
PMID:  17400307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurite RNA binding proteins are important for neurite growth, a process critical for neuronal development and regeneration after injury. It has been known that many RNA binding proteins undergo nucleocytoplasmic shuttling but how their nucleocytoplasmic distributions are regulated during neurite growth has not been well explored. Here we found that the polypyrimidine tract binding protein (PTB) was exported from the nucleus and accumulated at growing neurite terminals upon activation of the PKA pathway in PC12 cells in a PKA-target Ser16-dependent manner. RNA interference (RNAi) of PTB significantly disrupted the neurite growth. We then examined the role of cytoplasmic PTB in relation to mRNAs involved in neurite growth. We found that PTB was preferentially associated with the beta-actin mRNA transcripts in cytoplasmic fractions. RNAi of PTB reduced neurite accumulation of the endogenous actin proteins. It is thus likely that, during PKA-induced neurite growth, PTB is relocalized through Ser16 phosphorylation to the cytoplasm where it is associated with beta-actin mRNA and is critical for the mRNA localization to neurites.
Authors:
Shumei Ma; Guodong Liu; Yuan Sun; Jiuyong Xie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-22
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1773     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-07-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  912-23     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Medicine, University of Manitoba, 420 BMSB, 730 William Ave., Winnipeg, Canada MB R3E 3J7.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics,  metabolism
Active Transport, Cell Nucleus / physiology
Animals
Cell Nucleus / genetics,  metabolism*
Cyclic AMP-Dependent Protein Kinases / metabolism*
Cytoplasm / metabolism*
Neurites / enzymology*
PC12 Cells
Phosphorylation
Polypyrimidine Tract-Binding Protein / antagonists & inhibitors,  genetics,  metabolism*
Protein Processing, Post-Translational / physiology*
RNA, Messenger / genetics,  metabolism
RNA, Small Interfering / genetics
Rats
Chemical
Reg. No./Substance:
0/Actins; 0/RNA, Messenger; 0/RNA, Small Interfering; 139076-35-0/Polypyrimidine Tract-Binding Protein; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases

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