Document Detail


Reliable enzyme-linked immunosorbent assay for the determination of soybean proteins in processed foods.
MedLine Citation:
PMID:  18642843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Among allergenic foods, soybean is known as a food causing adverse reactions in allergenic patients. To clarify the validity of labeling, the specific and sensitive detection method for the analysis of the soybean protein would be necessary. The p34 protein, originally characterized to be p34 as an oil-body associated protein in soybean, has been identified as one of the major allergenic proteins and named Gly m Bd 30K. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of the soybean protein in processed foods was developed using polyclonal antibodies raised against p34 as a soybean marker protein and the specific extraction buffer for extract. The developed sandwich ELISA method was highly specific for the soybean protein. The limit of detection (LOD) and the limit of quantification (LOQ) of the developed ELISA were 0.47 ng/mL (equivalent to 0.19 microg/g in foods) and 0.94 ng/mL (equivalent to 0.38 microg/g in foods), respectively. The recovery ranged from 87.7 to 98.7%, whereas the intra- and interassay coefficients of variation were less than 4.2 and 7.5%, respectively. This study showed that the developed ELISA method is a specific, precise, and reliable tool for the quantitative analysis of the soybean protein in processed foods.
Authors:
Naoki Morishita; Kumiko Kamiya; Takashi Matsumoto; Shinobu Sakai; Reiko Teshima; Atsuo Urisu; Tatsuya Moriyama; Tadashi Ogawa; Hiroshi Akiyama; Fumiki Morimatsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-19
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6818-24     Citation Subset:  IM    
Affiliation:
R&D Center, Nippon Meat Packers, Inc., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis
Antigens, Plant / analysis
Enzyme-Linked Immunosorbent Assay / methods*
Food Analysis / methods*
Food Handling
Food Hypersensitivity / prevention & control
Food Labeling
Sensitivity and Specificity
Soybean Proteins / analysis*
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Plant; 0/Soybean Proteins; 147388-26-9/P34 protein, Glycine max

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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