Document Detail


Reliable detection of dead microbial cells by using fluorescent hydrazides.
MedLine Citation:
PMID:  20038683     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.
Authors:
Claude Saint-Ruf; Corinne Cordier; Jérome Mégret; Ivan Matic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-28
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-24     Completed Date:  2010-05-18     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1674-8     Citation Subset:  IM    
Affiliation:
INSERM U571, Faculté de Médecine Paris Descartes, Université René Descartes, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / metabolism*
Bacteriological Techniques / methods*
Fluorescent Dyes / metabolism*
Microbial Viability*
Staining and Labeling / methods*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

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