Document Detail

Reliable detection of dead microbial cells by using fluorescent hydrazides.
MedLine Citation:
PMID:  20038683     Owner:  NLM     Status:  MEDLINE    
We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.
Claude Saint-Ruf; Corinne Cordier; Jérome Mégret; Ivan Matic
Related Documents :
19562213 - Detection of drug-induced cellular changes using confocal raman spectroscopy on pattern...
20638423 - Development of a flow-through microscopic multitesting system for parallel monitoring o...
4063443 - Age-related change in numbers of other interstitial cells in testes of adult men: evide...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-28
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-24     Completed Date:  2010-05-18     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1674-8     Citation Subset:  IM    
INSERM U571, Faculté de Médecine Paris Descartes, Université René Descartes, 156 rue de Vaugirard, 75730 Paris Cedex 15, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacteria / metabolism*
Bacteriological Techniques / methods*
Fluorescent Dyes / metabolism*
Microbial Viability*
Staining and Labeling / methods*
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling prote...
Next Document:  Evaluation of Lactobacillus sobrius/L. amylovorus as a new microbial marker of pig manure.