| Reliable detection of dead microbial cells by using fluorescent hydrazides. | |
| | |
MedLine Citation:
|
PMID: 20038683 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms. |
| | |
Authors:
|
Claude Saint-Ruf; Corinne Cordier; Jérome Mégret; Ivan Matic |
Related Documents
:
|
21472123 - Glutamate reduces experimental intestinal hyperpermeability and facilitates glutamine s... 20628533 - Detecting single bacterial cells through optical resonances in microdroplets. 21355953 - Downregulation of the b-cell receptor signaling component cd79b in plasma cell myeloma:... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-02-24 Completed Date: 2010-05-18 Revised Date: 2010-09-02 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1674-8 Citation Subset: IM |
Affiliation:
|
INSERM U571, Faculté de Médecine Paris Descartes, Université René Descartes, 156 rue de Vaugirard, 75730 Paris Cedex 15, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
metabolism* Bacteriological Techniques / methods* Fluorescent Dyes / metabolism* Microbial Viability* Staining and Labeling / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling prote...
Next Document: Evaluation of Lactobacillus sobrius/amylovorus as a new microbial marker of pig manure.